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| | ==Cryo-EM structure of mouse TRPV3 in complex with 2-Aminoethoxydiphenyl borate (2-APB)== | | ==Cryo-EM structure of mouse TRPV3 in complex with 2-Aminoethoxydiphenyl borate (2-APB)== |
| - | <StructureSection load='6dvy' size='340' side='right'caption='[[6dvy]], [[Resolution|resolution]] 4.00Å' scene=''> | + | <SX load='6dvy' size='340' side='right' viewer='molstar' caption='[[6dvy]], [[Resolution|resolution]] 4.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6dvy]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DVY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DVY FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6dvy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DVY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6DVY FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FZ4:2-aminoethyl+diphenylborinate'>FZ4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Trpv3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FZ4:2-diphenylboranyloxyethanamine'>FZ4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dvy OCA], [http://pdbe.org/6dvy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dvy RCSB], [http://www.ebi.ac.uk/pdbsum/6dvy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dvy ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6dvy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dvy OCA], [https://pdbe.org/6dvy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6dvy RCSB], [https://www.ebi.ac.uk/pdbsum/6dvy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6dvy ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/TRPV3_MOUSE TRPV3_MOUSE]] Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen) (By similarity). | + | [https://www.uniprot.org/uniprot/TRPV3_MOUSE TRPV3_MOUSE] Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen) (By similarity). |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6dvy" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6dvy" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Ion channels 3D structures|Ion channels 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| - | </StructureSection> | + | </SX> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: McGoldrick, L L]] | + | [[Category: McGoldrick LL]] |
| - | [[Category: Singh, A K]] | + | [[Category: Singh AK]] |
| - | [[Category: Sobolevsky, A I]] | + | [[Category: Sobolevsky AI]] |
| - | [[Category: Calcium channel]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Trp channel]]
| + | |
| - | [[Category: Trpv3]]
| + | |
| Structural highlights
Function
TRPV3_MOUSE Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. Is a negative regulator of hair growth and cycling: TRPV3-coupled signaling suppresses keratinocyte proliferation in hair follicles and induces apoptosis and premature hair follicle regression (catagen) (By similarity).
Publication Abstract from PubMed
Transient receptor potential vanilloid subfamily member 3 (TRPV3) channel plays a crucial role in skin physiology and pathophysiology. Mutations in TRPV3 are associated with various skin diseases, including Olmsted syndrome, atopic dermatitis, and rosacea. Here we present the cryo-electron microscopy structures of full-length mouse TRPV3 in the closed apo and agonist-bound open states. The agonist binds three allosteric sites distal to the pore. Channel opening is accompanied by conformational changes in both the outer pore and the intracellular gate. The gate is formed by the pore-lining S6 helices that undergo local alpha-to-pi helical transitions, elongate, rotate, and splay apart in the open state. In the closed state, the shorter S6 segments are entirely alpha-helical, expose their nonpolar surfaces to the pore, and hydrophobically seal the ion permeation pathway. These findings further illuminate TRP channel activation and can aid in the design of drugs for the treatment of inflammatory skin conditions, itch, and pain.
Structure and gating mechanism of the transient receptor potential channel TRPV3.,Singh AK, McGoldrick LL, Sobolevsky AI Nat Struct Mol Biol. 2018 Sep;25(9):805-813. doi: 10.1038/s41594-018-0108-7. Epub, 2018 Aug 20. PMID:30127359[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Singh AK, McGoldrick LL, Sobolevsky AI. Structure and gating mechanism of the transient receptor potential channel TRPV3. Nat Struct Mol Biol. 2018 Sep;25(9):805-813. doi: 10.1038/s41594-018-0108-7. Epub, 2018 Aug 20. PMID:30127359 doi:http://dx.doi.org/10.1038/s41594-018-0108-7
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