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| | <StructureSection load='2vye' size='340' side='right'caption='[[2vye]], [[Resolution|resolution]] 4.10Å' scene=''> | | <StructureSection load='2vye' size='340' side='right'caption='[[2vye]], [[Resolution|resolution]] 4.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2vye]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Geoka Geoka]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VYE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2VYE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2vye]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Geobacillus_kaustophilus_HTA426 Geobacillus kaustophilus HTA426]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VYE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VYE FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2vyf|2vyf]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 4.1Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vye OCA], [http://pdbe.org/2vye PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vye RCSB], [http://www.ebi.ac.uk/pdbsum/2vye PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vye ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2vye FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vye OCA], [https://pdbe.org/2vye PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2vye RCSB], [https://www.ebi.ac.uk/pdbsum/2vye PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2vye ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q5KU75_GEOKA Q5KU75_GEOKA] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Geoka]] | + | [[Category: Escherichia coli]] |
| | + | [[Category: Geobacillus kaustophilus HTA426]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Hsiao, C D]] | + | [[Category: Hsiao CD]] |
| - | [[Category: Lo, Y H]] | + | [[Category: Lo YH]] |
| - | [[Category: Sun, Y J]] | + | [[Category: Sun YJ]] |
| - | [[Category: Tsai, K L]] | + | [[Category: Tsai KL]] |
| - | [[Category: Atp-binding]]
| + | |
| - | [[Category: Dna replication]]
| + | |
| - | [[Category: Dnac]]
| + | |
| - | [[Category: Helicase]]
| + | |
| - | [[Category: Hydrolase]]
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| - | [[Category: Hydrolase-dna complex]]
| + | |
| - | [[Category: Nucleotide-binding]]
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| Structural highlights
Function
Q5KU75_GEOKA
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
DNA helicases are motor proteins that play essential roles in DNA replication, repair and recombination. In the replicative hexameric helicase, the fundamental reaction is the unwinding of duplex DNA; however, our understanding of this function remains vague due to insufficient structural information. Here, we report two crystal structures of the DnaB-family replicative helicase from Geobacillus kaustophilus HTA426 (GkDnaC) in the apo-form and bound to single-stranded DNA (ssDNA). The GkDnaC-ssDNA complex structure reveals that three symmetrical basic grooves on the interior surface of the hexamer individually encircle ssDNA. The ssDNA-binding pockets in this structure are directed toward the N-terminal domain collar of the hexameric ring, thus orienting the ssDNA toward the DnaG primase to facilitate the synthesis of short RNA primers. These findings provide insight into the mechanism of ssDNA binding and provide a working model to establish a novel mechanism for DNA translocation at the replication fork.
The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA.,Lo YH, Tsai KL, Sun YJ, Chen WT, Huang CY, Hsiao CD Nucleic Acids Res. 2008 Dec 15. PMID:19074952[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Lo YH, Tsai KL, Sun YJ, Chen WT, Huang CY, Hsiao CD. The crystal structure of a replicative hexameric helicase DnaC and its complex with single-stranded DNA. Nucleic Acids Res. 2008 Dec 15. PMID:19074952 doi:gkn999
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