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| | <StructureSection load='6igj' size='340' side='right'caption='[[6igj]], [[Resolution|resolution]] 1.50Å' scene=''> | | <StructureSection load='6igj' size='340' side='right'caption='[[6igj]], [[Resolution|resolution]] 1.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6igj]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IGJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IGJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6igj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IGJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6IGJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.501Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6igj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6igj OCA], [http://pdbe.org/6igj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6igj RCSB], [http://www.ebi.ac.uk/pdbsum/6igj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6igj ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6igj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6igj OCA], [https://pdbe.org/6igj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6igj RCSB], [https://www.ebi.ac.uk/pdbsum/6igj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6igj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FT_ARATH FT_ARATH]] Probable component of the mobile flower-promoting signal (floral stimulus or florigen). Promotes the transition from vegetative growth to flowering. Required for 'SEPALLATA3' (SEP3) and 'FRUITFULL' (FUL) accumulation in mature rosette leaves. Seems to acts in parallel with 'LEAFY' to induce flowering by regulating 'APETALA1'. May play a role in both the autonomous and the long-day flowering pathways.<ref>PMID:16155177</ref> <ref>PMID:16099979</ref> <ref>PMID:16099980</ref> | + | [https://www.uniprot.org/uniprot/FT_ARATH FT_ARATH] Probable component of the mobile flower-promoting signal (floral stimulus or florigen). Promotes the transition from vegetative growth to flowering. Required for 'SEPALLATA3' (SEP3) and 'FRUITFULL' (FUL) accumulation in mature rosette leaves. Seems to acts in parallel with 'LEAFY' to induce flowering by regulating 'APETALA1'. May play a role in both the autonomous and the long-day flowering pathways.<ref>PMID:16155177</ref> <ref>PMID:16099979</ref> <ref>PMID:16099980</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Inaba, K]] | + | [[Category: Inaba K]] |
| - | [[Category: Kanehara, K]] | + | [[Category: Kanehara K]] |
| - | [[Category: Nakamura, Y]] | + | [[Category: Nakamura Y]] |
| - | [[Category: Watanabe, S]] | + | [[Category: Watanabe S]] |
| - | [[Category: Flowering control]]
| + | |
| - | [[Category: Plant protein]]
| + | |
| Structural highlights
Function
FT_ARATH Probable component of the mobile flower-promoting signal (floral stimulus or florigen). Promotes the transition from vegetative growth to flowering. Required for 'SEPALLATA3' (SEP3) and 'FRUITFULL' (FUL) accumulation in mature rosette leaves. Seems to acts in parallel with 'LEAFY' to induce flowering by regulating 'APETALA1'. May play a role in both the autonomous and the long-day flowering pathways.[1] [2] [3]
Publication Abstract from PubMed
Arabidopsis FLOWERING LOCUS T (FT) is a pivotal component of florigen, a long-range mobile flowering signal. Here, we determined the 1.0 A-resolution crystal structure of FT, a significantly higher-resolution crystal structure of FT than previously reported one (2.6 A). The present crystallographic studies revealed 4 alternative configurations with the precise location of the surrounding water molecules. Using this structural data, computational docking simulation predicted the putative binding sites for phosphatidylcholine (PC), an endogenous ligand that interacts with FT to modulate flowering time. In vitro reconstitution of the lipid-protein interaction showed that mutations at two of the predicted sites significantly compromised the lipid binding ability of FT. In planta, one of the mutant FT proteins significantly affected FT function in flowering, emphasizing the involvement of PC binding in modulating FT function. Our structural, biochemical, and transgenic analyses reveal the molecular mechanism of PC binding in FT-mediated flowering time control.
High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering.,Nakamura Y, Lin YC, Watanabe S, Liu YC, Katsuyama K, Kanehara K, Inaba K iScience. 2019 Nov 22;21:577-586. doi: 10.1016/j.isci.2019.10.045. Epub 2019 Oct , 26. PMID:31726375[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Teper-Bamnolker P, Samach A. The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves. Plant Cell. 2005 Oct;17(10):2661-75. Epub 2005 Sep 9. PMID:16155177 doi:http://dx.doi.org/tpc.105.035766
- ↑ Abe M, Kobayashi Y, Yamamoto S, Daimon Y, Yamaguchi A, Ikeda Y, Ichinoki H, Notaguchi M, Goto K, Araki T. FD, a bZIP protein mediating signals from the floral pathway integrator FT at the shoot apex. Science. 2005 Aug 12;309(5737):1052-6. PMID:16099979 doi:http://dx.doi.org/309/5737/1052
- ↑ Wigge PA, Kim MC, Jaeger KE, Busch W, Schmid M, Lohmann JU, Weigel D. Integration of spatial and temporal information during floral induction in Arabidopsis. Science. 2005 Aug 12;309(5737):1056-9. PMID:16099980 doi:http://dx.doi.org/309/5737/1056
- ↑ Nakamura Y, Lin YC, Watanabe S, Liu YC, Katsuyama K, Kanehara K, Inaba K. High-Resolution Crystal Structure of Arabidopsis FLOWERING LOCUS T Illuminates Its Phospholipid-Binding Site in Flowering. iScience. 2019 Nov 22;21:577-586. doi: 10.1016/j.isci.2019.10.045. Epub 2019 Oct , 26. PMID:31726375 doi:http://dx.doi.org/10.1016/j.isci.2019.10.045
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