6tob
From Proteopedia
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==Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF== | ==Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF== | ||
| - | <StructureSection load='6tob' size='340' side='right'caption='[[6tob | + | <StructureSection load='6tob' size='340' side='right'caption='[[6tob]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6tob]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TOB OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6tob]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis Mycobacterium tuberculosis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TOB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TOB FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tob OCA], [https://pdbe.org/6tob PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tob RCSB], [https://www.ebi.ac.uk/pdbsum/6tob PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tob ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/IHF_MYCTU IHF_MYCTU] A nucleoid-associated protein (NAP) required for septum formation and normal cell division as well as for DNA segregation. Binds about 135 sites across the chromosome, most of which are genes involved in virulence; most DNA-binding sites are immediately upstream of transcription start sites. When mIHF is depleted most of the genes are down-regulated (PubMed:30242166). Binds supercoiled and linear dsDNA in a concentration-dependent manner, probably non-sequence specifically. Binding compacts DNA, protecting it from degradation. Initial binding to supercoiled DNA opens it fully, followed by bending and compaction. Bends and thus compacts linear DNA (PubMed:23922883). Binds DNA via 2 sites, forms left-handed loops on linear DNA; at low concentrations unwinds larger cosmids (42.6 kb) then collapses and condenses DNA as protein levels rise. Forms mostly left-handed loops on condensing cosmid DNA (PubMed:31846718).<ref>PMID:23922883</ref> <ref>PMID:30242166</ref> <ref>PMID:31846718</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Mycobacterium tuberculosis]] |
| - | [[Category: | + | [[Category: Herrmann T]] |
| - | + | ||
Current revision
Structural and DNA Binding Properties of Mycobacterial Integration Host Factor mIHF
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