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| | <StructureSection load='1zle' size='340' side='right'caption='[[1zle]], [[Resolution|resolution]] 1.90Å' scene=''> | | <StructureSection load='1zle' size='340' side='right'caption='[[1zle]], [[Resolution|resolution]] 1.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1zle]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Pyrenophora_tritici-repentis Pyrenophora tritici-repentis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZLE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1zle]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrenophora_tritici-repentis Pyrenophora tritici-repentis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZLE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZLE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1zld|1zld]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1zle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zle OCA], [https://pdbe.org/1zle PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1zle RCSB], [https://www.ebi.ac.uk/pdbsum/1zle PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1zle ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1zle FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1zle OCA], [http://pdbe.org/1zle PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1zle RCSB], [http://www.ebi.ac.uk/pdbsum/1zle PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1zle ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/P78737_9PLEO P78737_9PLEO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Pyrenophora tritici-repentis]] | | [[Category: Pyrenophora tritici-repentis]] |
| - | [[Category: Ciuffetti, L M]] | + | [[Category: Ciuffetti LM]] |
| - | [[Category: Karplus, P A]] | + | [[Category: Karplus PA]] |
| - | [[Category: Manning, V A]] | + | [[Category: Manning VA]] |
| - | [[Category: Sarma, G N]] | + | [[Category: Sarma GN]] |
| - | [[Category: Beta-sandwich]]
| + | |
| - | [[Category: Rgd-motif]]
| + | |
| - | [[Category: Toxin]]
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| Structural highlights
Function
P78737_9PLEO
Publication Abstract from PubMed
Tan spot of wheat (Triticum aestivum), caused by the fungus Pyrenophora tritici-repentis, has significant agricultural and economic impact. Ptr ToxA (ToxA), the first discovered proteinaceous host-selective toxin, is produced by certain P. tritici-repentis races and is necessary and sufficient to cause cell death in sensitive wheat cultivars. We present here the high-resolution crystal structure of ToxA in two different crystal forms, providing four independent views of the protein. ToxA adopts a single-domain, beta-sandwich fold of novel topology. Mapping of the existing mutation data onto the structure supports the hypothesized importance of an Arg-Gly-Asp (RGD) and surrounding sequence. Its occurrence in a single, solvent-exposed loop in the protein suggests that it is directly involved in recognition events required for ToxA action. Furthermore, the ToxA structure reveals a surprising similarity with the classic mammalian RGD-containing domain, the fibronectin type III (FnIII) domain: the two topologies are related by circular permutation. The similar topologies and the positional conservation of the RGD-containing loop raises the possibility that ToxA is distantly related to mammalian FnIII proteins and that to gain entry it binds to an integrin-like receptor in the plant host.
Structure of Ptr ToxA: an RGD-containing host-selective toxin from Pyrenophora tritici-repentis.,Sarma GN, Manning VA, Ciuffetti LM, Karplus PA Plant Cell. 2005 Nov;17(11):3190-202. Epub 2005 Oct 7. PMID:16214901[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sarma GN, Manning VA, Ciuffetti LM, Karplus PA. Structure of Ptr ToxA: an RGD-containing host-selective toxin from Pyrenophora tritici-repentis. Plant Cell. 2005 Nov;17(11):3190-202. Epub 2005 Oct 7. PMID:16214901 doi:tpc.105.034918
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