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| | ==Solution NMR structure of Asteropsin A from marine sponge Asteropus sp.== | | ==Solution NMR structure of Asteropsin A from marine sponge Asteropus sp.== |
| - | <StructureSection load='2lqa' size='340' side='right'caption='[[2lqa]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2lqa' size='340' side='right'caption='[[2lqa]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2lqa]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Asteropus Asteropus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LQA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2LQA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2lqa]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Asteropus Asteropus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2LQA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2LQA FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3q8j|3q8j]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PCA:PYROGLUTAMIC+ACID'>PCA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2lqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lqa OCA], [http://pdbe.org/2lqa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2lqa RCSB], [http://www.ebi.ac.uk/pdbsum/2lqa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2lqa ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2lqa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2lqa OCA], [https://pdbe.org/2lqa PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2lqa RCSB], [https://www.ebi.ac.uk/pdbsum/2lqa PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2lqa ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/I1SB10_9METZ I1SB10_9METZ] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Asteropus]] | | [[Category: Asteropus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bowling, J J]] | + | [[Category: Bowling JJ]] |
| - | [[Category: Hamann, M T]] | + | [[Category: Hamann MT]] |
| - | [[Category: Jung, J H]] | + | [[Category: Jung JH]] |
| - | [[Category: Li, H]] | + | [[Category: Li H]] |
| - | [[Category: Knottin]]
| + | |
| - | [[Category: Sponge]]
| + | |
| - | [[Category: Toxin]]
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| Structural highlights
Function
I1SB10_9METZ
Publication Abstract from PubMed
BACKGROUND: Herein we report the discovery of a cystine-crosslinked peptide from Porifera along with high-quality spatial details accompanied by the description of its unique effect on neuronal calcium influx. METHODS: Asteropsin A (ASPA) was isolated from the marine sponge Asteropus sp., and its structure was independently determined using X-ray crystallography (0.87A) and solution NMR spectroscopy. RESULTS: An N-terminal pyroglutamate modification, uncommon cis proline conformations, and absence of basic residues helped distinguish ASPA from other cystine-crosslinked knot peptides. ASPA enhanced Ca(2+) influx in murine cerebrocortical neuron cells following the addition of the Na(+) channel activator veratridine but did not modify the oscillation frequency or amplitude of neuronal Ca(2+) currents alone. Allosterism at neurotoxin site 2 was not observed, suggesting an alternative to the known Na(+) channel interaction. CONCLUSIONS: Together with a distinct biological activity, the origin of ASPA suggests a new subclass of cystine-rich knot peptides associated with Porifera. GENERAL SIGNIFICANCE: The discovery of ASPA represents a distinctive addition to an emerging subclass of cystine-crosslinked knot peptides from Porifera.
Asteropsin A: An unusual cystine-crosslinked peptide from porifera enhances neuronal Ca(2+) influx.,Li H, Bowling JJ, Fronczek FR, Hong J, Jabba SV, Murray TF, Ha NC, Hamann MT, Jung JH Biochim Biophys Acta. 2012 Nov 29;1830(3):2591-2599. doi:, 10.1016/j.bbagen.2012.11.015. PMID:23201194[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Li H, Bowling JJ, Fronczek FR, Hong J, Jabba SV, Murray TF, Ha NC, Hamann MT, Jung JH. Asteropsin A: An unusual cystine-crosslinked peptide from porifera enhances neuronal Ca(2+) influx. Biochim Biophys Acta. 2012 Nov 29;1830(3):2591-2599. doi:, 10.1016/j.bbagen.2012.11.015. PMID:23201194 doi:http://dx.doi.org/10.1016/j.bbagen.2012.11.015
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