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2var

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[[Image:2var.gif|left|200px]]<br />
 
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<applet load="2var" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="2var, resolution 2.10&Aring;" />
 
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'''CRYSTAL STRUCTURE OF SULFOLOBUS SOLFATARICUS 2-KETO-3-DEOXYGLUCONATE KINASE COMPLEXED WITH 2-KETO-3-DEOXYGLUCONATE'''<br />
 
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==About this Structure==
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==Crystal structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate==
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2VAR is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Sulfolobus_solfataricus Sulfolobus solfataricus] with KDG, KDF and ANP as [http://en.wikipedia.org/wiki/ligands ligands]. Active as [http://en.wikipedia.org/wiki/Fructokinase Fructokinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.4 2.7.1.4] Structure known Active Site: AC1. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=2VAR OCA].
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<StructureSection load='2var' size='340' side='right'caption='[[2var]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
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[[Category: Fructokinase]]
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== Structural highlights ==
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[[Category: Single protein]]
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<table><tr><td colspan='2'>[[2var]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharolobus_solfataricus Saccharolobus solfataricus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2VAR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2VAR FirstGlance]. <br>
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[[Category: Sulfolobus solfataricus]]
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
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[[Category: Bull, S.D.]]
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=KDF:3-DEOXY-ALPHA-D-ERYTHRO-HEX-2-ULOFURANOSONIC+ACID'>KDF</scene>, <scene name='pdbligand=KDG:2-KETO-3-DEOXYGLUCONATE'>KDG</scene></td></tr>
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[[Category: Danson, M.J.]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2var FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2var OCA], [https://pdbe.org/2var PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2var RCSB], [https://www.ebi.ac.uk/pdbsum/2var PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2var ProSAT]</span></td></tr>
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[[Category: Hough, D.W.]]
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</table>
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[[Category: Kerou, M.]]
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== Function ==
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[[Category: Lamble, H.J.]]
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[https://www.uniprot.org/uniprot/KDGK_SACS2 KDGK_SACS2] Involved in the degradation of glucose and galactose via the semi-phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) and 2-keto-3-deoxygalactonate (KDGal) to produce 2-keto-3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxy-6-phosphogalactonate (KDPGal), respectively.<ref>PMID:15869466</ref> <ref>PMID:16330030</ref> <ref>PMID:16794308</ref>
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[[Category: Potter, J.A.]]
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== Evolutionary Conservation ==
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[[Category: Taylor, G.L.]]
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[[Image:Consurf_key_small.gif|200px|right]]
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[[Category: Theodossis, A.]]
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Check<jmol>
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[[Category: ANP]]
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<jmolCheckbox>
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[[Category: KDF]]
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<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/va/2var_consurf.spt"</scriptWhenChecked>
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[[Category: KDG]]
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<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
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[[Category: 2- keto-3-deoxygluconate kinase]]
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<text>to colour the structure by Evolutionary Conservation</text>
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[[Category: 2- keto-3-deoxygluconate kinase kinase]]
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</jmolCheckbox>
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[[Category: kinase]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2var ConSurf].
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[[Category: pfkb family carbohydrate kinase]]
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<div style="clear:both"></div>
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[[Category: sulfolobus solfataricus]]
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<div style="background-color:#fffaf0;">
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[[Category: transferase]]
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== Publication Abstract from PubMed ==
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The hyperthermophilic archaeon Sulfolobus solfataricus grows optimally above 353 K and utilizes an unusual promiscuous nonphosphorylative Entner-Doudoroff pathway to metabolize both glucose and galactose. It has been proposed that a part-phosphorylative Entner-Doudoroff pathway occurs in parallel in S. solfataricus, in which the 2-keto-3-deoxygluconate kinase (KDGK) is promiscuous for both glucose and galactose metabolism. Recombinant S. solfataricus KDGK protein was expressed in Escherichia coli, purified and crystallized in 0.1 M sodium acetate pH 4.1 and 1.4 M NaCl. The crystal structure of apo S. solfataricus KDGK was solved by molecular replacement to a resolution of 2.0 A and a ternary complex with 2-keto-3-deoxygluconate (KDGlu) and an ATP analogue was resolved at 2.1 A. The complex suggests that the structural basis for the enzyme's ability to phosphorylate KDGlu and 2-keto-3-deoxygalactonate (KDGal) is derived from a subtle repositioning of residues that are conserved in homologous nonpromiscuous kinases.
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 18:53:57 2007''
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The structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase.,Potter JA, Kerou M, Lamble HJ, Bull SD, Hough DW, Danson MJ, Taylor GL Acta Crystallogr D Biol Crystallogr. 2008 Dec;64(Pt 12):1283-7. Epub 2008, Nov 18. PMID:19018105<ref>PMID:19018105</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 2var" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Fructokinase|Fructokinase]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Saccharolobus solfataricus]]
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[[Category: Bull SD]]
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[[Category: Danson MJ]]
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[[Category: Hough DW]]
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[[Category: Kerou M]]
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[[Category: Lamble HJ]]
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[[Category: Potter JA]]
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[[Category: Taylor GL]]
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[[Category: Theodossis A]]

Current revision

Crystal structure of Sulfolobus solfataricus 2-keto-3-deoxygluconate kinase complexed with 2-keto-3-deoxygluconate

PDB ID 2var

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