5f15

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<StructureSection load='5f15' size='340' side='right'caption='[[5f15]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
<StructureSection load='5f15' size='340' side='right'caption='[[5f15]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5f15]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cupmc Cupmc]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F15 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F15 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5f15]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Cupriavidus_metallidurans_CH34 Cupriavidus metallidurans CH34]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F15 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F15 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=5TR:[(2~{E},6~{E},10~{E},14~{E},18~{Z},22~{E},26~{Z},30~{E},34~{E},38~{E})-3,7,11,15,19,23,27,31,35,39,43-UNDECAMETHYLTETRATETRACONTA-2,6,10,14,18,22,26,30,34,38,42-UNDECAENYL]+DIHYDROGEN+PHOSPHATE'>5TR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5ezm|5ezm]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5TR:[(2~{E},6~{E},10~{E},14~{E},18~{Z},22~{E},26~{Z},30~{E},34~{E},38~{E})-3,7,11,15,19,23,27,31,35,39,43-UNDECAMETHYLTETRATETRACONTA-2,6,10,14,18,22,26,30,34,38,42-UNDECAENYL]+DIHYDROGEN+PHOSPHATE'>5TR</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=MPG:[(Z)-OCTADEC-9-ENYL]+(2R)-2,3-BIS(OXIDANYL)PROPANOATE'>MPG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Rmet_4828, AmT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=266264 CUPMC])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f15 OCA], [https://pdbe.org/5f15 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f15 RCSB], [https://www.ebi.ac.uk/pdbsum/5f15 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f15 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f15 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f15 OCA], [http://pdbe.org/5f15 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f15 RCSB], [http://www.ebi.ac.uk/pdbsum/5f15 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f15 ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/Q1LDT6_CUPMC Q1LDT6_CUPMC]
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Polymyxins are antibiotics used in the last line of defense to combat multidrug-resistant infections by Gram-negative bacteria. Polymyxin resistance arises through charge modification of the bacterial outer membrane with the attachment of the cationic sugar 4-amino-4-deoxy-l-arabinose to lipid A, a reaction catalyzed by the integral membrane lipid-to-lipid glycosyltransferase 4-amino-4-deoxy-L-arabinose transferase (ArnT). Here, we report crystal structures of ArnT from Cupriavidus metallidurans, alone and in complex with the lipid carrier undecaprenyl phosphate, at 2.8 and 3.2 angstrom resolution, respectively. The structures show cavities for both lipidic substrates, which converge at the active site. A structural rearrangement occurs on undecaprenyl phosphate binding, which stabilizes the active site and likely allows lipid A binding. Functional mutagenesis experiments based on these structures suggest a mechanistic model for ArnT family enzymes.
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Structures of aminoarabinose transferase ArnT suggest a molecular basis for lipid A glycosylation.,Petrou VI, Herrera CM, Schultz KM, Clarke OB, Vendome J, Tomasek D, Banerjee S, Rajashankar KR, Belcher Dufrisne M, Kloss B, Kloppmann E, Rost B, Klug CS, Trent MS, Shapiro L, Mancia F Science. 2016 Feb 5;351(6273):608-12. doi: 10.1126/science.aad1172. PMID:26912703<ref>PMID:26912703</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5f15" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cupmc]]
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[[Category: Cupriavidus metallidurans CH34]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Banerjee, S]]
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[[Category: Banerjee S]]
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[[Category: Clarke, O B]]
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[[Category: Clarke OB]]
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[[Category: Mancia, F]]
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[[Category: Mancia F]]
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[[Category: NYCOMPS, New York Consortium on Membrane Protein Structure]]
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[[Category: Petrou VI]]
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[[Category: Petrou, V I]]
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[[Category: Rajashankar KR]]
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[[Category: Rajashankar, K R]]
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[[Category: Tomasek D]]
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[[Category: Tomasek, D]]
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[[Category: Gt-c fold]]
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[[Category: Lipid glycosyltransferase]]
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[[Category: Membrane protein]]
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[[Category: New york consortium on membrane protein structure]]
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[[Category: Nycomp]]
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[[Category: Psi-biology]]
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[[Category: Structural genomic]]
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[[Category: Transferase]]
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[[Category: Undecaprenyl phosphate]]
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Current revision

Crystal Structure of ArnT from Cupriavidus metallidurans bound to Undecaprenyl phosphate

PDB ID 5f15

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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