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| | <StructureSection load='5ji1' size='340' side='right'caption='[[5ji1]], [[Resolution|resolution]] 2.25Å' scene=''> | | <StructureSection load='5ji1' size='340' side='right'caption='[[5ji1]], [[Resolution|resolution]] 2.25Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ji1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JI1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JI1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ji1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JI1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5JI1 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.25Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5jhw|5jhw]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MPD:(4S)-2-METHYL-2,4-PENTANEDIOL'>MPD</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mstn, Gdf8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ji1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ji1 OCA], [https://pdbe.org/5ji1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ji1 RCSB], [https://www.ebi.ac.uk/pdbsum/5ji1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ji1 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ji1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ji1 OCA], [http://pdbe.org/5ji1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ji1 RCSB], [http://www.ebi.ac.uk/pdbsum/5ji1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ji1 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GDF8_MOUSE GDF8_MOUSE]] Acts specifically as a negative regulator of skeletal muscle growth. | + | [https://www.uniprot.org/uniprot/GDF8_MOUSE GDF8_MOUSE] Acts specifically as a negative regulator of skeletal muscle growth. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Thompson, T B]] | + | [[Category: Thompson TB]] |
| - | [[Category: Walker, R G]] | + | [[Category: Walker RG]] |
| - | [[Category: Cytokine]]
| + | |
| - | [[Category: Gdf8]]
| + | |
| - | [[Category: Ligand]]
| + | |
| - | [[Category: Myostatin]]
| + | |
| - | [[Category: Tgfbeta]]
| + | |
| Structural highlights
Function
GDF8_MOUSE Acts specifically as a negative regulator of skeletal muscle growth.
Publication Abstract from PubMed
BACKGROUND: Growth/differentiation factor 8 (GDF8) and GDF11 are two highly similar members of the transforming growth factor beta (TGFbeta) family. While GDF8 has been recognized as a negative regulator of muscle growth and differentiation, there are conflicting studies on the function of GDF11 and whether GDF11 has beneficial effects on age-related dysfunction. To address whether GDF8 and GDF11 are functionally identical, we compared their signaling and structural properties. RESULTS: Here we show that, despite their high similarity, GDF11 is a more potent activator of SMAD2/3 and signals more effectively through the type I activin-like receptor kinase receptors ALK4/5/7 than GDF8. Resolution of the GDF11:FS288 complex, apo-GDF8, and apo-GDF11 crystal structures reveals unique properties of both ligands, specifically in the type I receptor binding site. Lastly, substitution of GDF11 residues into GDF8 confers enhanced activity to GDF8. CONCLUSIONS: These studies identify distinctive structural features of GDF11 that enhance its potency, relative to GDF8; however, the biological consequences of these differences remain to be determined.
Structural basis for potency differences between GDF8 and GDF11.,Walker RG, Czepnik M, Goebel EJ, McCoy JC, Vujic A, Cho M, Oh J, Aykul S, Walton KL, Schang G, Bernard DJ, Hinck AP, Harrison CA, Martinez-Hackert E, Wagers AJ, Lee RT, Thompson TB BMC Biol. 2017 Mar 3;15(1):19. doi: 10.1186/s12915-017-0350-1. PMID:28257634[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Walker RG, Czepnik M, Goebel EJ, McCoy JC, Vujic A, Cho M, Oh J, Aykul S, Walton KL, Schang G, Bernard DJ, Hinck AP, Harrison CA, Martinez-Hackert E, Wagers AJ, Lee RT, Thompson TB. Structural basis for potency differences between GDF8 and GDF11. BMC Biol. 2017 Mar 3;15(1):19. doi: 10.1186/s12915-017-0350-1. PMID:28257634 doi:http://dx.doi.org/10.1186/s12915-017-0350-1
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