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| | <StructureSection load='5xa5' size='340' side='right'caption='[[5xa5]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='5xa5' size='340' side='right'caption='[[5xa5]], [[Resolution|resolution]] 1.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5xa5]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Caeel Caeel]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XA5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XA5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5xa5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Caenorhabditis_elegans Caenorhabditis elegans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XA5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5XA5 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">hmp-1, R13H4.4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL]), hmp-2, K05C4.6 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6239 CAEEL])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xa5 OCA], [http://pdbe.org/5xa5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xa5 RCSB], [http://www.ebi.ac.uk/pdbsum/5xa5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xa5 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5xa5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xa5 OCA], [https://pdbe.org/5xa5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5xa5 RCSB], [https://www.ebi.ac.uk/pdbsum/5xa5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5xa5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HMP1_CAEEL HMP1_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation (PubMed:9531567). Required for proper localization of other junctional components, such as pac-1 (PubMed:25938815).<ref>PMID:25938815</ref> <ref>PMID:9531567</ref> [[http://www.uniprot.org/uniprot/HMP2_CAEEL HMP2_CAEEL]] Required for cell migration during body enclosure and cell shape changes during body elongation.<ref>PMID:9531567</ref> | + | [https://www.uniprot.org/uniprot/HMP1_CAEEL HMP1_CAEEL] Required for cell migration during body enclosure and cell shape changes during body elongation (PubMed:9531567). Required for proper localization of other junctional components, such as pac-1 (PubMed:25938815).<ref>PMID:25938815</ref> <ref>PMID:9531567</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Stable tissue integrity during embryonic development relies on the function of the cadherin.catenin complex (CCC). The Caenorhabditis elegans CCC is a useful paradigm for analyzing in vivo requirements for specific interactions among the core components of the CCC, and it provides a unique opportunity to examine evolutionarily conserved mechanisms that govern the interaction between alpha- and beta-catenin. HMP-1, unlike its mammalian homolog alpha-catenin, is constitutively monomeric, and its binding affinity for HMP-2/beta-catenin is higher than that of alpha-catenin for beta-catenin. A crystal structure shows that the HMP-1.HMP-2 complex forms a five-helical bundle structure distinct from the structure of the mammalian alpha-catenin.beta-catenin complex. Deletion analysis based on the crystal structure shows that the first helix of HMP-1 is necessary for binding HMP-2 avidly in vitro and for efficient recruitment of HMP-1 to adherens junctions in embryos. HMP-2 Ser-47 and Tyr-69 flank its binding interface with HMP-1, and we show that phosphomimetic mutations at these two sites decrease binding affinity of HMP-1 to HMP-2 by 40-100-fold in vitro. In vivo experiments using HMP-2 S47E and Y69E mutants showed that they are unable to rescue hmp-2(zu364) mutants, suggesting that phosphorylation of HMP-2 on Ser-47 and Tyr-69 could be important for regulating CCC formation in C. elegans Our data provide novel insights into how cadherin-dependent cell-cell adhesion is modulated in metazoans by conserved elements as well as features unique to specific organisms.
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| - | Cell-cell adhesion in metazoans relies on evolutionarily conserved features of the alpha-catenin.beta-catenin-binding interface.,Shao X, Kang H, Loveless T, Lee GR, Seok C, Weis WI, Choi HJ, Hardin J J Biol Chem. 2017 Oct 6;292(40):16477-16490. doi: 10.1074/jbc.M117.795567. Epub, 2017 Aug 25. PMID:28842483<ref>PMID:28842483</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 5xa5" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Caeel]] | + | [[Category: Caenorhabditis elegans]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Choi, H J]] | + | [[Category: Choi HJ]] |
| - | [[Category: Hardin, J]] | + | [[Category: Hardin J]] |
| - | [[Category: Kang, H]] | + | [[Category: Kang H]] |
| - | [[Category: Shao, X]] | + | [[Category: Shao X]] |
| - | [[Category: Weis, W I]] | + | [[Category: Weis WI]] |
| - | [[Category: Cell adhesion]]
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| - | [[Category: Five-helix bundle]]
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