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| ==Solution Structure of the Carboxyl Terminal Domain of the Ciliary Neurotrophic Factor Receptor== | | ==Solution Structure of the Carboxyl Terminal Domain of the Ciliary Neurotrophic Factor Receptor== |
- | <StructureSection load='1uc6' size='340' side='right'caption='[[1uc6]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''> | + | <StructureSection load='1uc6' size='340' side='right'caption='[[1uc6]]' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[1uc6]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UC6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UC6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1uc6]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UC6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UC6 FirstGlance]. <br> |
- | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uc6 OCA], [http://pdbe.org/1uc6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uc6 RCSB], [http://www.ebi.ac.uk/pdbsum/1uc6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uc6 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uc6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uc6 OCA], [https://pdbe.org/1uc6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uc6 RCSB], [https://www.ebi.ac.uk/pdbsum/1uc6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uc6 ProSAT]</span></td></tr> |
| </table> | | </table> |
| == Function == | | == Function == |
- | [[http://www.uniprot.org/uniprot/CNTFR_HUMAN CNTFR_HUMAN]] Binds to CNTF. The alpha subunit provides the receptor specificity. | + | [https://www.uniprot.org/uniprot/CNTFR_HUMAN CNTFR_HUMAN] Binds to CNTF. The alpha subunit provides the receptor specificity. |
| == Evolutionary Conservation == | | == Evolutionary Conservation == |
| [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
- | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: He, W]] | + | [[Category: He W]] |
- | [[Category: Ip, N Y]] | + | [[Category: Ip NY]] |
- | [[Category: Ke, G]] | + | [[Category: Ke G]] |
- | [[Category: Man, D]] | + | [[Category: Man D]] |
- | [[Category: Smith, D K]] | + | [[Category: Smith DK]] |
- | [[Category: Sze, K H]] | + | [[Category: Sze KH]] |
- | [[Category: Zhu, G]] | + | [[Category: Zhu G]] |
- | [[Category: Ciliary neurotrophic factor]]
| + | |
- | [[Category: Cytokine]]
| + | |
- | [[Category: Cytokine receptor]]
| + | |
- | [[Category: Fibronectin type iii domain-like topology]]
| + | |
- | [[Category: Leukemia inhibitory factor]]
| + | |
- | [[Category: Protein binding]]
| + | |
- | [[Category: Seven beta-strand]]
| + | |
- | [[Category: Two anti-parallel beta-sheet]]
| + | |
| Structural highlights
Function
CNTFR_HUMAN Binds to CNTF. The alpha subunit provides the receptor specificity.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The functional receptor complex of ciliary neurotrophic factor (CNTF), a member of the gp130 family of cytokines, is composed of CNTF, the CNTF receptor alpha (CNTFR), gp130, and the leukemia inhibitory factor receptor (LIFR). However, the nature of the receptor-mediated interactions in this complex has not yet been resolved. To address this issue we have determined the solution structure of the C-terminal or BC domain of CNTFR and studied the interactions of CNTFR with LIFR and gp130. We reported previously that the membrane distal cytokine-binding domain (CBD1) of LIFR could interact in vitro with soluble CNTFR (sCNTFR) in the absence of CNTF. Here we show that the CBD of human gp130 can also bind in vitro to sCNTFR in the absence of CNTF. In addition, the gp130 CBD could compete with the LIFR CBD1 for the binding of sCNTFR. Substitution of residues in the gp130 CBD, the LIFR CBD1, and the CNTFR BC domain that are expected to be involved in receptor-receptor interactions significantly reduced their interactions. An NMR chemical shift perturbation study of the interaction between the BC domains of CNTFR and gp130 further mapped the interaction surface. These data suggest that both the gp130 CBD and the LIFR CBD1 interact with CNTFR in a similar way and provide insights into the nature of the CNTF receptor complex.
Solution structure of the C-terminal domain of the ciliary neurotrophic factor (CNTF) receptor and ligand free associations among components of the CNTF receptor complex.,Man D, He W, Sze KH, Gong K, Smith DK, Zhu G, Ip NY J Biol Chem. 2003 Jun 27;278(26):23285-94. Epub 2003 Apr 21. PMID:12707266[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Man D, He W, Sze KH, Gong K, Smith DK, Zhu G, Ip NY. Solution structure of the C-terminal domain of the ciliary neurotrophic factor (CNTF) receptor and ligand free associations among components of the CNTF receptor complex. J Biol Chem. 2003 Jun 27;278(26):23285-94. Epub 2003 Apr 21. PMID:12707266 doi:http://dx.doi.org/10.1074/jbc.M301976200
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