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| | ==Solution structure of human Liver Expressed Antimicrobial Peptide 2== | | ==Solution structure of human Liver Expressed Antimicrobial Peptide 2== |
| - | <StructureSection load='2l1q' size='340' side='right'caption='[[2l1q]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2l1q' size='340' side='right'caption='[[2l1q]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2l1q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L1Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L1Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2l1q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L1Q FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">LEAP2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l1q OCA], [http://pdbe.org/2l1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l1q RCSB], [http://www.ebi.ac.uk/pdbsum/2l1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l1q ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l1q OCA], [https://pdbe.org/2l1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l1q RCSB], [https://www.ebi.ac.uk/pdbsum/2l1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l1q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LEAP2_HUMAN LEAP2_HUMAN]] Has an antimicrobial activity.<ref>PMID:20845358</ref> | + | [https://www.uniprot.org/uniprot/LEAP2_HUMAN LEAP2_HUMAN] Has an antimicrobial activity.<ref>PMID:20845358</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | <jmolCheckbox> | | <jmolCheckbox> |
| | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l1/2l1q_consurf.spt"</scriptWhenChecked> | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/l1/2l1q_consurf.spt"</scriptWhenChecked> |
| - | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked> |
| | <text>to colour the structure by Evolutionary Conservation</text> | | <text>to colour the structure by Evolutionary Conservation</text> |
| | </jmolCheckbox> | | </jmolCheckbox> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Clark, R J]] | + | [[Category: Clark RJ]] |
| - | [[Category: Antimicrobial protein]]
| + | |
| - | [[Category: Disulfide]]
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| - | [[Category: Leap-2]]
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| - | [[Category: Polypeptide]]
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| Structural highlights
Function
LEAP2_HUMAN Has an antimicrobial activity.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Human liver-expressed antimicrobial peptide 2 (LEAP-2) is a cationic antimicrobial peptide (CAMP) believed to have a protective role against bacterial infection. Little is known about the structure-activity relationships of LEAP-2 or its mechanism of action. In this study we describe the structure of LEAP-2, analyze its interaction with model membranes, and relate them to the antimicrobial activity of the peptide. The structure of LEAP-2, determined by NMR spectroscopy, reveals a compact central core with disorder at the N and C termini. The core comprises a beta-hairpin and a 3(10)- helix that are braced by disulfide bonds between Cys17-28 and Cys23-33 and further stabilized by a network of hydrogen bonds. Membrane-affinity studies show that LEAP-2 membrane binding is governed by electrostatic attractions, which are sensitive to ionic strength. Truncation studies show that the C-terminal region of LEAP-2 is irrelevant for membrane binding, whereas the N-terminal (hydrophobic domain) and core regions (cationic domain) are essential. Bacterial-growth-inhibition assays reveal that the antimicrobial activity of LEAP-2 correlates with membrane affinity. Interestingly, the native and reduced forms of LEAP-2 have similar membrane affinity and antimicrobial activities; this suggests that disulfide bonds are not essential for the bactericidal activity. This study reveals that LEAP-2 has a novel fold for a CAMP and suggests that although LEAP-2 exhibits antimicrobial activity under low-salt conditions, there is likely to be another physiological role for the peptide.
Structural and functional analysis of human liver-expressed antimicrobial peptide 2.,Henriques ST, Tan CC, Craik DJ, Clark RJ Chembiochem. 2010 Oct 18;11(15):2148-57. doi: 10.1002/cbic.201000400. PMID:20845358[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Henriques ST, Tan CC, Craik DJ, Clark RJ. Structural and functional analysis of human liver-expressed antimicrobial peptide 2. Chembiochem. 2010 Oct 18;11(15):2148-57. doi: 10.1002/cbic.201000400. PMID:20845358 doi:http://dx.doi.org/10.1002/cbic.201000400
- ↑ Henriques ST, Tan CC, Craik DJ, Clark RJ. Structural and functional analysis of human liver-expressed antimicrobial peptide 2. Chembiochem. 2010 Oct 18;11(15):2148-57. doi: 10.1002/cbic.201000400. PMID:20845358 doi:http://dx.doi.org/10.1002/cbic.201000400
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