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| | ==Solution Structure of Cytotoxic T-Lymphocyte Antigent-2(Ctla protein), Crammer at pH 6.0== | | ==Solution Structure of Cytotoxic T-Lymphocyte Antigent-2(Ctla protein), Crammer at pH 6.0== |
| - | <StructureSection load='2l95' size='340' side='right'caption='[[2l95]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='2l95' size='340' side='right'caption='[[2l95]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2l95]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L95 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L95 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2l95]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L95 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L95 FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CER, CER-RA, DMEL_CG10460 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l95 OCA], [http://pdbe.org/2l95 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l95 RCSB], [http://www.ebi.ac.uk/pdbsum/2l95 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l95 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l95 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l95 OCA], [https://pdbe.org/2l95 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l95 RCSB], [https://www.ebi.ac.uk/pdbsum/2l95 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l95 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/A1ZBK7_DROME A1ZBK7_DROME] |
| - | Drosophila melanogaster crammer is a novel cathepsin inhibitor that is involved in LTM (long-term memory) formation. The mechanism by which the inhibitory activity is regulated remains unclear. In the present paper we have shown that the oligomeric state of crammer is pH dependent. At neutral pH, crammer is predominantly dimeric in vitro as a result of disulfide bond formation, and is monomeric at acidic pH. Our inhibition assay shows that monomeric crammer, not disulfide-bonded dimer, is a strong competitive inhibitor of cathepsin L. Crammer is a monomeric molten globule in acidic solution, a condition that is similar to the environment in the lysosome where crammer is probably located. Upon binding to cathepsin L, however, crammer undergoes a molten globule-to-ordered structural transition. Using high-resolution NMR spectroscopy, we have shown that a cysteine-to-serine point mutation at position 72 (C72S) renders crammer monomeric at pH 6.0 and that the structure of the C72S variant highly resembles that of wild-type crammer in complex with cathepsin L at pH 4.0. We have determined the first solution structure of propeptide-like protease inhibitor in its active form and examined in detail using a variety of spectroscopic methods the folding properties of crammer in order to delineate its biomolecular recognition of cathepsin.
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| - | A molten globule-to-ordered structure transition of Drosophila melanogaster crammer is required for its ability to inhibit cathepsin.,Tseng TS, Cheng CS, Chen DJ, Shih MF, Liu YN, Hsu ST, Lyu PC Biochem J. 2012 Mar 15;442(3):563-72. PMID:22150223<ref>PMID:22150223</ref>
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| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| - | </div>
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| - | <div class="pdbe-citations 2l95" style="background-color:#fffaf0;"></div>
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| - | == References ==
| + | |
| - | <references/>
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cheng, C S]] | + | [[Category: Cheng CS]] |
| - | [[Category: Liu, Y N]] | + | [[Category: Liu YN]] |
| - | [[Category: Lyu, P C]] | + | [[Category: Lyu PC]] |
| - | [[Category: Tseng, T S]] | + | [[Category: Tseng TS]] |
| - | [[Category: Crammer]]
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| - | [[Category: Cysteine proteinase inhibitor]]
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| - | [[Category: Hydrolase]]
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| - | [[Category: Intrinsic disorder p ctla-2-like protein]]
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