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| | <StructureSection load='4oxs' size='340' side='right'caption='[[4oxs]], [[Resolution|resolution]] 2.80Å' scene=''> | | <StructureSection load='4oxs' size='340' side='right'caption='[[4oxs]], [[Resolution|resolution]] 2.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4oxs]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Magmm Magmm]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4OXS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4oxs]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Magnetococcus_marinus_MC-1 Magnetococcus marinus MC-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4OXS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4OXS FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=2CV:HEGA-10'>2CV</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.8Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4bha|4bha]], [[4cbc|4cbc]], [[4cal|4cal]], [[4ox1|4ox1]], [[4ox4|4ox4]], [[4oxg|4oxg]], [[4oxh|4oxh]], [[4oxt|4oxt]], [[4oxu|4oxu]], [[4oy0|4oy0]], [[4oy1|4oy1]], [[4oxz|4oxz]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2CV:HEGA-10'>2CV</scene>, <scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mmc1_0798 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=156889 MAGMM])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4oxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxs OCA], [https://pdbe.org/4oxs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4oxs RCSB], [https://www.ebi.ac.uk/pdbsum/4oxs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4oxs ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4oxs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4oxs OCA], [http://pdbe.org/4oxs PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4oxs RCSB], [http://www.ebi.ac.uk/pdbsum/4oxs PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4oxs ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0L5S6_MAGMM A0L5S6_MAGMM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Magmm]] | + | [[Category: Magnetococcus marinus MC-1]] |
| - | [[Category: Bagneris, C]] | + | [[Category: Bagneris C]] |
| - | [[Category: Naylor, C E]] | + | [[Category: Naylor CE]] |
| - | [[Category: Wallace, B A]] | + | [[Category: Wallace BA]] |
| - | [[Category: Channel blocking compound]]
| + | |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Pore]]
| + | |
| - | [[Category: Sodium channel]]
| + | |
| - | [[Category: Transport protein]]
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| Structural highlights
Function
A0L5S6_MAGMM
Publication Abstract from PubMed
Voltage-gated sodium channels are important targets for the development of pharmaceutical drugs, because mutations in different human sodium channel isoforms have causal relationships with a range of neurological and cardiovascular diseases. In this study, functional electrophysiological studies show that the prokaryotic sodium channel from Magnetococcus marinus (NavMs) binds and is inhibited by eukaryotic sodium channel blockers in a manner similar to the human Nav1.1 channel, despite millions of years of divergent evolution between the two types of channels. Crystal complexes of the NavMs pore with several brominated blocker compounds depict a common antagonist binding site in the cavity, adjacent to lipid-facing fenestrations proposed to be the portals for drug entry. In silico docking studies indicate the full extent of the blocker binding site, and electrophysiology studies of NavMs channels with mutations at adjacent residues validate the location. These results suggest that the NavMs channel can be a valuable tool for screening and rational design of human drugs.
Prokaryotic NavMs channel as a structural and functional model for eukaryotic sodium channel antagonism.,Bagneris C, DeCaen PG, Naylor CE, Pryde DC, Nobeli I, Clapham DE, Wallace BA Proc Natl Acad Sci U S A. 2014 May 21. pii: 201406855. PMID:24850863[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bagneris C, DeCaen PG, Naylor CE, Pryde DC, Nobeli I, Clapham DE, Wallace BA. Prokaryotic NavMs channel as a structural and functional model for eukaryotic sodium channel antagonism. Proc Natl Acad Sci U S A. 2014 May 21. pii: 201406855. PMID:24850863 doi:http://dx.doi.org/10.1073/pnas.1406855111
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