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| | ==Design of a novel cyclic peptide that alleviates symptoms in a murine model of inflammatory bowel disease== | | ==Design of a novel cyclic peptide that alleviates symptoms in a murine model of inflammatory bowel disease== |
| - | <StructureSection load='5vav' size='340' side='right'caption='[[5vav]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5vav' size='340' side='right'caption='[[5vav]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5vav]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VAV FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5vav]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Helianthus_annuus Helianthus annuus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VAV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VAV FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vfw|5vfw]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vav OCA], [http://pdbe.org/5vav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vav RCSB], [http://www.ebi.ac.uk/pdbsum/5vav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vav ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vav OCA], [https://pdbe.org/5vav PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vav RCSB], [https://www.ebi.ac.uk/pdbsum/5vav PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vav ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| | + | [[Category: Helianthus annuus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Caceres, C Cobos]] | + | [[Category: Cobos Caceres C]] |
| - | [[Category: Daly, N L]] | + | [[Category: Daly NL]] |
| - | [[Category: De novo protein]]
| + | |
| - | [[Category: Inflammatory bowel disease]]
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| Structural highlights
Publication Abstract from PubMed
Inflammatory bowel diseases (IBDs) are a set of complex and debilitating diseases, for which there is no satisfactory treatment. Recent studies have shown that small peptides show promise for reducing inflammation in models of IBD. However, these small peptides are likely to be unstable and rapidly cleared from the circulation, and therefore, if not modified for better stability, represent non-viable drug leads. We hypothesized that improving the stability of these peptides by grafting them into a stable cyclic peptide scaffold may enhance their therapeutic potential. Using this approach, we have designed a novel cyclic peptide, which comprises a small bioactive peptide from the annexin A1 protein grafted into a sunflower trypsin inhibitor cyclic scaffold. We used native chemical ligation to synthesize the grafted cyclic peptide. This engineered cyclic peptide maintained the overall fold of the naturally occurring cyclic peptide, was more effective at reducing inflammation in a mouse model of acute colitis than the bioactive peptide alone, and showed enhanced stability in human serum. Our findings suggest that the use of cyclic peptides as structural backbones offers a promising approach for the treatment of IBD and potentially other chronic inflammatory conditions.
An engineered cyclic peptide alleviates symptoms of inflammation in a murine model of inflammatory bowel disease.,Cobos Caceres C, Bansal PS, Navarro S, Wilson D, Don L, Giacomin P, Loukas A, Daly NL J Biol Chem. 2017 May 4. pii: jbc.M117.779215. doi: 10.1074/jbc.M117.779215. PMID:28473469[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cobos Caceres C, Bansal PS, Navarro S, Wilson D, Don L, Giacomin P, Loukas A, Daly NL. An engineered cyclic peptide alleviates symptoms of inflammation in a murine model of inflammatory bowel disease. J Biol Chem. 2017 May 4. pii: jbc.M117.779215. doi: 10.1074/jbc.M117.779215. PMID:28473469 doi:http://dx.doi.org/10.1074/jbc.M117.779215
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