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| | <StructureSection load='5vgr' size='340' side='right'caption='[[5vgr]], [[Resolution|resolution]] 2.10Å' scene=''> | | <StructureSection load='5vgr' size='340' side='right'caption='[[5vgr]], [[Resolution|resolution]] 2.10Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5vgr]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VGR OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VGR FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5vgr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VGR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VGR FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.096Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ATL3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vgr OCA], [http://pdbe.org/5vgr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vgr RCSB], [http://www.ebi.ac.uk/pdbsum/5vgr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vgr ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vgr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vgr OCA], [https://pdbe.org/5vgr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vgr RCSB], [https://www.ebi.ac.uk/pdbsum/5vgr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vgr ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Disease == | | == Disease == |
| - | [[http://www.uniprot.org/uniprot/ATLA3_HUMAN ATLA3_HUMAN]] Hereditary sensory and autonomic neuropathy type 1. The disease is caused by mutations affecting the gene represented in this entry. | + | [https://www.uniprot.org/uniprot/ATLA3_HUMAN ATLA3_HUMAN] Hereditary sensory and autonomic neuropathy type 1. The disease is caused by mutations affecting the gene represented in this entry. |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ATLA3_HUMAN ATLA3_HUMAN]] GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.<ref>PMID:18270207</ref> <ref>PMID:19665976</ref> | + | [https://www.uniprot.org/uniprot/ATLA3_HUMAN ATLA3_HUMAN] GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.<ref>PMID:18270207</ref> <ref>PMID:19665976</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Donnell, J P.O]] | + | [[Category: O'Donnell JP]] |
| - | [[Category: Sondermann, H]] | + | [[Category: Sondermann H]] |
| - | [[Category: G protein]]
| + | |
| - | [[Category: Gtpase]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Membrane fusion]]
| + | |
| Structural highlights
Disease
ATLA3_HUMAN Hereditary sensory and autonomic neuropathy type 1. The disease is caused by mutations affecting the gene represented in this entry.
Function
ATLA3_HUMAN GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis.[1] [2]
Publication Abstract from PubMed
The endoplasmic reticulum (ER) forms a branched, dynamic membrane tubule network that is vital for cellular function. Branching arises from membrane fusion facilitated by the GTPase atlastin (ATL). Many metazoan genomes encode for three ATL isoforms that appear to fulfill partially redundant function despite differences in their intrinsic GTPase activity and localization within the ER; however, the underlying mechanistic differences between the isoforms are poorly understood. Here, we identify discrete temporal steps in the catalytic cycle for the two most dissimilar isoforms, ATL1 and ATL3, revealing an overall conserved progression of molecular events from nucleotide binding and hydrolysis to ATL dimerization and phosphate release. A crystal structure of ATL3 suggests a mechanism for the displacement of the catalytic Mg2+ ion following guanosine triphosphate (GTP) hydrolysis. Together, the data extend the mechanistic framework for how GTP hydrolysis drives conformational changes in ATL and how the cycle is reset for subsequent rounds of catalysis.
Timing and Reset Mechanism of GTP Hydrolysis-Driven Conformational Changes of Atlastin.,O'Donnell JP, Cooley RB, Kelly CM, Miller K, Andersen OS, Rusinova R, Sondermann H Structure. 2017 Jul 5;25(7):997-1010.e4. doi: 10.1016/j.str.2017.05.007. Epub, 2017 Jun 9. PMID:28602821[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rismanchi N, Soderblom C, Stadler J, Zhu PP, Blackstone C. Atlastin GTPases are required for Golgi apparatus and ER morphogenesis. Hum Mol Genet. 2008 Jun 1;17(11):1591-604. doi: 10.1093/hmg/ddn046. Epub 2008 Feb, 12. PMID:18270207 doi:10.1093/hmg/ddn046
- ↑ Hu J, Shibata Y, Zhu PP, Voss C, Rismanchi N, Prinz WA, Rapoport TA, Blackstone C. A class of dynamin-like GTPases involved in the generation of the tubular ER network. Cell. 2009 Aug 7;138(3):549-61. PMID:19665976 doi:S0092-8674(09)00628-X
- ↑ O'Donnell JP, Cooley RB, Kelly CM, Miller K, Andersen OS, Rusinova R, Sondermann H. Timing and Reset Mechanism of GTP Hydrolysis-Driven Conformational Changes of Atlastin. Structure. 2017 Jul 5;25(7):997-1010.e4. doi: 10.1016/j.str.2017.05.007. Epub, 2017 Jun 9. PMID:28602821 doi:http://dx.doi.org/10.1016/j.str.2017.05.007
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