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| | <StructureSection load='5vn7' size='340' side='right'caption='[[5vn7]], [[Resolution|resolution]] 2.70Å' scene=''> | | <StructureSection load='5vn7' size='340' side='right'caption='[[5vn7]], [[Resolution|resolution]] 2.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5vn7]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Halsa Halsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VN7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VN7 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5vn7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum_NRC-1 Halobacterium salinarum NRC-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VN7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5VN7 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5vn9|5vn9]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=LYR:N~6~-[(2Z,4E,6E,8E)-3,7-DIMETHYL-9-(2,6,6-TRIMETHYLCYCLOHEX-1-EN-1-YL)NONA-2,4,6,8-TETRAENYL]LYSINE'>LYR</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bop, VNG_1467G ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64091 HALSA])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5vn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vn7 OCA], [https://pdbe.org/5vn7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5vn7 RCSB], [https://www.ebi.ac.uk/pdbsum/5vn7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5vn7 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vn7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vn7 OCA], [http://pdbe.org/5vn7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vn7 RCSB], [http://www.ebi.ac.uk/pdbsum/5vn7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vn7 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA]] Light-driven proton pump. | + | [https://www.uniprot.org/uniprot/BACR_HALSA BACR_HALSA] Light-driven proton pump. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Halsa]] | + | [[Category: Halobacterium salinarum NRC-1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Borshchevskiy, V]] | + | [[Category: Borshchevskiy V]] |
| - | [[Category: Cherezov, V]] | + | [[Category: Cherezov V]] |
| - | [[Category: Ishchenko, A]] | + | [[Category: Ishchenko A]] |
| - | [[Category: Kuklin, A]] | + | [[Category: Kuklin A]] |
| - | [[Category: Lee, S C]] | + | [[Category: Lee SC]] |
| - | [[Category: Mishin, A]] | + | [[Category: Mishin A]] |
| - | [[Category: Peng, L]] | + | [[Category: Peng L]] |
| - | [[Category: Vlasov, A]] | + | [[Category: Vlasov A]] |
| - | [[Category: Zhang, Q]] | + | [[Category: Zhang Q]] |
| - | [[Category: Zinovev, E]] | + | [[Category: Zinovev E]] |
| - | [[Category: Membrane protein]]
| + | |
| - | [[Category: Retinal protein]]
| + | |
| Structural highlights
Function
BACR_HALSA Light-driven proton pump.
Publication Abstract from PubMed
Lipidic cubic phase (LCP) has been widely recognized as a promising membrane-mimicking matrix for biophysical studies of membrane proteins and their crystallization in a lipidic environment. Application of this material to a wide variety of membrane proteins, however, is hindered due to a limited number of available host lipids, mostly monoacylglycerols (MAGs). Here, we designed, synthesized and characterized a series of chemically stable lipids resistant to hydrolysis, with properties complementary to the widely used MAGs. In order to assess their potential to serve as host lipids for crystallization, we characterized the phase properties and lattice parameters of mesophases made of two most promising lipids at a variety of different conditions by polarized light microscopy and small-angle X-ray scattering. Both lipids showed remarkable chemical stability and an extended LCP region in the phase diagram covering a wide range of temperatures down to 4 degrees C. One of these lipids has been used for crystallization and structure determination of a prototypical membrane protein bacteriorhodopsin at 4 degrees C and 20 degrees C.
Chemically Stable Lipids for Membrane Protein Crystallization.,Ishchenko A, Peng L, Zinovev E, Vlasov A, Lee SC, Kuklin A, Mishin A, Borshchevskiy V, Zhang Q, Cherezov V Cryst Growth Des. 2017 Jun 7;17(6):3502-3511. doi: 10.1021/acs.cgd.7b00458. Epub , 2017 May 12. PMID:29290736[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ishchenko A, Peng L, Zinovev E, Vlasov A, Lee SC, Kuklin A, Mishin A, Borshchevskiy V, Zhang Q, Cherezov V. Chemically Stable Lipids for Membrane Protein Crystallization. Cryst Growth Des. 2017 Jun 7;17(6):3502-3511. doi: 10.1021/acs.cgd.7b00458. Epub , 2017 May 12. PMID:29290736 doi:http://dx.doi.org/10.1021/acs.cgd.7b00458
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