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1bdn

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(New page: 200px<br /> <applet load="1bdn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1bdn, resolution 2.600&Aring;" /> '''CRYSTAL LATTICE PA...)
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[[Image:1bdn.gif|left|200px]]<br />
 
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<applet load="1bdn" size="450" color="white" frame="true" align="right" spinBox="true"
 
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caption="1bdn, resolution 2.600&Aring;" />
 
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'''CRYSTAL LATTICE PACKING IS IMPORTANT IN DETERMINING THE BEND OF A DNA DODECAMER CONTAINING AN ADENINE TRACT'''<br />
 
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==Overview==
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==CRYSTAL LATTICE PACKING IS IMPORTANT IN DETERMINING THE BEND OF A DNA DODECAMER CONTAINING AN ADENINE TRACT==
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The crystal structure of a DNA duplex dodecamer d(CGCAAAAATGCG) and its, complementary strand has been determined at 2.6-A resolution. Although our, goal was to deduce the structural features of the static bending of the, helical axis exhibited by adenine-tract structures in solution, we, conclude that the overall bend of 20 degrees in the direction of the major, groove observed here arises from the forces associated with crystal, packing. An isomorphous dodecamer brominated on one strand provides, experimental evidence that this asymmetric sequence is positioned in two, orientations in the crystal lattice that are related by a 180 degrees, rotation around the pseudodyad axis of the sequence. The bend in these two, differently positioned DNA molecules depends on their orientation in the, crystal, not on their sequence. As with previously determined structures, containing adenine tracts, the adenine and thymine base pairs are highly, propeller twisted. The N-6 of the adenine comes within hydrogen bonding, distance of the O-4 of thymine one step down the helix, facilitating the, formation of a series of bifurcated hydrogen bonds within the adenine, tract. The adenine tract is relatively straight and the bending is, localized outside this region.
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<StructureSection load='1bdn' size='340' side='right'caption='[[1bdn]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
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== Structural highlights ==
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==About this Structure==
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<table><tr><td colspan='2'>[[1bdn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1BDN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1BDN FirstGlance]. <br>
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1BDN is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/ ] with MG as [http://en.wikipedia.org/wiki/ligand ligand]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1BDN OCA].
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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==Reference==
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1bdn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1bdn OCA], [https://pdbe.org/1bdn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1bdn RCSB], [https://www.ebi.ac.uk/pdbsum/1bdn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1bdn ProSAT]</span></td></tr>
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Crystal lattice packing is important in determining the bend of a DNA dodecamer containing an adenine tract., DiGabriele AD, Sanderson MR, Steitz TA, Proc Natl Acad Sci U S A. 1989 Mar;86(6):1816-20. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=2928304 2928304]
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</table>
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[[Category: Protein complex]]
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__TOC__
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[[Category: DiGabriele, A.D.]]
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</StructureSection>
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[[Category: Sanderson, M.R.]]
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[[Category: Large Structures]]
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[[Category: Steitz, T.A.]]
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[[Category: DiGabriele AD]]
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[[Category: MG]]
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[[Category: Sanderson MR]]
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[[Category: b-dna]]
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[[Category: Steitz TA]]
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[[Category: double helix]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 12:38:04 2007''
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Current revision

CRYSTAL LATTICE PACKING IS IMPORTANT IN DETERMINING THE BEND OF A DNA DODECAMER CONTAINING AN ADENINE TRACT

PDB ID 1bdn

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