6b0c

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==KLP10A-AMPPNP in complex with curved tubulin and a microtubule==
==KLP10A-AMPPNP in complex with curved tubulin and a microtubule==
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<StructureSection load='6b0c' size='340' side='right'caption='[[6b0c]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
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<SX load='6b0c' size='340' side='right' viewer='molstar' caption='[[6b0c]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6b0c]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B0C FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6b0c]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6B0C FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.51&#8491;</td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Klp10A, CG1453 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b0c OCA], [http://pdbe.org/6b0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b0c RCSB], [http://www.ebi.ac.uk/pdbsum/6b0c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b0c ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6b0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b0c OCA], [https://pdbe.org/6b0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6b0c RCSB], [https://www.ebi.ac.uk/pdbsum/6b0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6b0c ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/KI10A_DROME KI10A_DROME]] Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux".<ref>PMID:14681690</ref> [[http://www.uniprot.org/uniprot/F2Z5B2_PIG F2Z5B2_PIG]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU003505]
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[https://www.uniprot.org/uniprot/TBA1B_PIG TBA1B_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Kinesin-13s constitute a distinct group within the kinesin superfamily of motor proteins that promote microtubule depolymerization and lack motile activity. The molecular mechanism by which kinesin-13s depolymerize microtubules and are adapted to perform a seemingly very different activity from other kinesins is still unclear. To address this issue, here we report the near atomic resolution cryo-electron microscopy (cryo-EM) structures of Drosophila melanogaster kinesin-13 KLP10A protein constructs bound to curved or straight tubulin in different nucleotide states. These structures show how nucleotide induced conformational changes near the catalytic site are coupled with movement of the kinesin-13-specific loop-2 to induce tubulin curvature leading to microtubule depolymerization. The data highlight a modular structure that allows similar kinesin core motor-domains to be used for different functions, such as motility or microtubule depolymerization.
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Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s.,Benoit MPMH, Asenjo AB, Sosa H Nat Commun. 2018 Apr 25;9(1):1662. doi: 10.1038/s41467-018-04044-8. PMID:29695795<ref>PMID:29695795</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 6b0c" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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*[[Tubulin|Tubulin]]
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*[[Kinesin 3D Structures|Kinesin 3D Structures]]
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== References ==
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*[[Tubulin 3D Structures|Tubulin 3D Structures]]
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<references/>
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__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Drome]]
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[[Category: Drosophila melanogaster]]
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Sus scrofa]]
[[Category: Sus scrofa]]
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[[Category: Asenjo, A B]]
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[[Category: Asenjo AB]]
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[[Category: Benoit, M P.M H]]
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[[Category: Benoit MPMH]]
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[[Category: Sosa, H]]
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[[Category: Sosa H]]
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[[Category: Depolymerization]]
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[[Category: Kinesin 13]]
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[[Category: Microtubule]]
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[[Category: Motor protein-structural protein complex]]
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[[Category: Tubulin]]
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Current revision

KLP10A-AMPPNP in complex with curved tubulin and a microtubule

6b0c, resolution 3.51Å

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