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| | <StructureSection load='6coh' size='340' side='right'caption='[[6coh]], [[Resolution|resolution]] 2.37Å' scene=''> | | <StructureSection load='6coh' size='340' side='right'caption='[[6coh]], [[Resolution|resolution]] 2.37Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6coh]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6COH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6COH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6coh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6COH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6COH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HBX:BENZALDEHYDE'>HBX</scene>, <scene name='pdbligand=MXN:(2R)-HYDROXY(PHENYL)ETHANENITRILE'>MXN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.367Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HNL, MES5, At5g10300, F18D22_70 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HBX:BENZALDEHYDE'>HBX</scene>, <scene name='pdbligand=MXN:(2R)-HYDROXY(PHENYL)ETHANENITRILE'>MXN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/(R)-mandelonitrile_lyase (R)-mandelonitrile lyase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.2.10 4.1.2.10] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6coh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6coh OCA], [https://pdbe.org/6coh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6coh RCSB], [https://www.ebi.ac.uk/pdbsum/6coh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6coh ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6coh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6coh OCA], [http://pdbe.org/6coh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6coh RCSB], [http://www.ebi.ac.uk/pdbsum/6coh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6coh ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/HNL_ARATH HNL_ARATH]] Involved in cyanogenesis, the release of HCN from injured tissues (By similarity). Displays R-selective hydroxynitrile lyase activity. Also accepts nitromethane (MeNO2) as a donor in a reaction with aromatic aldehydes to yield (R)-beta-nitro alcohols.<ref>PMID:18467465</ref> <ref>PMID:19433222</ref> <ref>PMID:21439333</ref> <ref>PMID:17907254</ref> | + | [https://www.uniprot.org/uniprot/HNL_ARATH HNL_ARATH] Involved in cyanogenesis, the release of HCN from injured tissues (By similarity). Displays R-selective hydroxynitrile lyase activity. Also accepts nitromethane (MeNO2) as a donor in a reaction with aromatic aldehydes to yield (R)-beta-nitro alcohols.<ref>PMID:18467465</ref> <ref>PMID:19433222</ref> <ref>PMID:21439333</ref> <ref>PMID:17907254</ref> |
| | | | |
| | ==See Also== | | ==See Also== |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Arath]] | + | [[Category: Arabidopsis thaliana]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Desrouleaux, R]] | + | [[Category: Desrouleaux R]] |
| - | [[Category: Jones, B J]] | + | [[Category: Jones BJ]] |
| - | [[Category: Kazlauskas, R J]] | + | [[Category: Kazlauskas RJ]] |
| - | [[Category: Alpha beta hydrolase globular hydroxynitrile lyase]]
| + | |
| - | [[Category: Lyase]]
| + | |
| Structural highlights
Function
HNL_ARATH Involved in cyanogenesis, the release of HCN from injured tissues (By similarity). Displays R-selective hydroxynitrile lyase activity. Also accepts nitromethane (MeNO2) as a donor in a reaction with aromatic aldehydes to yield (R)-beta-nitro alcohols.[1] [2] [3] [4]
See Also
References
- ↑ Yang Y, Xu R, Ma CJ, Vlot AC, Klessig DF, Pichersky E. Inactive methyl indole-3-acetic acid ester can be hydrolyzed and activated by several esterases belonging to the AtMES esterase family of Arabidopsis. Plant Physiol. 2008 Jul;147(3):1034-45. doi: 10.1104/pp.108.118224. Epub 2008 May, 8. PMID:18467465 doi:http://dx.doi.org/10.1104/pp.108.118224
- ↑ Guterl JK, Andexer JN, Sehl T, von Langermann J, Frindi-Wosch I, Rosenkranz T, Fitter J, Gruber K, Kragl U, Eggert T, Pohl M. Uneven twins: comparison of two enantiocomplementary hydroxynitrile lyases with alpha/beta-hydrolase fold. J Biotechnol. 2009 May 20;141(3-4):166-73. doi: 10.1016/j.jbiotec.2009.03.010., Epub 2009 Mar 27. PMID:19433222 doi:http://dx.doi.org/10.1016/j.jbiotec.2009.03.010
- ↑ Fuhshuku K, Asano Y. Synthesis of (R)-beta-nitro alcohols catalyzed by R-selective hydroxynitrile lyase from Arabidopsis thaliana in the aqueous-organic biphasic system. J Biotechnol. 2011 May 20;153(3-4):153-9. doi: 10.1016/j.jbiotec.2011.03.011., Epub 2011 Mar 23. PMID:21439333 doi:http://dx.doi.org/10.1016/j.jbiotec.2011.03.011
- ↑ Andexer J, von Langermann J, Mell A, Bocola M, Kragl U, Eggert T, Pohl M. An R-selective hydroxynitrile lyase from Arabidopsis thaliana with an alpha/beta-hydrolase fold. Angew Chem Int Ed Engl. 2007;46(45):8679-81. PMID:17907254 doi:http://dx.doi.org/10.1002/anie.200701455
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