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| | <StructureSection load='1uw6' size='340' side='right'caption='[[1uw6]], [[Resolution|resolution]] 2.20Å' scene=''> | | <StructureSection load='1uw6' size='340' side='right'caption='[[1uw6]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1uw6]] is a 20 chain structure with sequence from [http://en.wikipedia.org/wiki/Great_pond_snail Great pond snail]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UW6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UW6 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1uw6]] is a 20 chain structure with sequence from [https://en.wikipedia.org/wiki/Lymnaea_stagnalis Lymnaea stagnalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UW6 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UW6 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NCT:(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE'>NCT</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1i9b|1i9b]], [[1uv6|1uv6]], [[1ux2|1ux2]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NCT:(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE'>NCT</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uw6 OCA], [http://pdbe.org/1uw6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uw6 RCSB], [http://www.ebi.ac.uk/pdbsum/1uw6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uw6 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uw6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uw6 OCA], [https://pdbe.org/1uw6 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uw6 RCSB], [https://www.ebi.ac.uk/pdbsum/1uw6 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uw6 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/ACHP_LYMST ACHP_LYMST]] Binds to acetylcholine. Modulates neuronal synaptic transmission. | + | [https://www.uniprot.org/uniprot/ACHP_LYMST ACHP_LYMST] Binds to acetylcholine. Modulates neuronal synaptic transmission. |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Great pond snail]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Brejc, K]] | + | [[Category: Lymnaea stagnalis]] |
| - | [[Category: Celie, P H.N]] | + | [[Category: Brejc K]] |
| - | [[Category: Dijk, W J.Van]] | + | [[Category: Celie PHN]] |
| - | [[Category: Rossum-fikkert, S E.Van]]
| + | [[Category: Sixma TK]] |
| - | [[Category: Sixma, T K]] | + | [[Category: Smit AB]] |
| - | [[Category: Smit, A B]] | + | [[Category: Van Dijk WJ]] |
| - | [[Category: Acetylcholine]] | + | [[Category: Van Rossum-fikkert SE]] |
| - | [[Category: Glycoprotein]] | + | |
| - | [[Category: Igg fold]]
| + | |
| - | [[Category: Nicotine]]
| + | |
| - | [[Category: Pentamer]]
| + | |
| Structural highlights
Function
ACHP_LYMST Binds to acetylcholine. Modulates neuronal synaptic transmission.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Nicotinic acetylcholine receptors are prototypes for the pharmaceutically important family of pentameric ligand-gated ion channels. Here we present atomic resolution structures of nicotine and carbamylcholine binding to AChBP, a water-soluble homolog of the ligand binding domain of nicotinic receptors and their family members, GABAA, GABAC, 5HT3 serotonin, and glycine receptors. Ligand binding is driven by enthalpy and is accompanied by conformational changes in the ligand binding site. Residues in the binding site contract around the ligand, with the largest movement in the C loop. As expected, the binding is characterized by substantial aromatic and hydrophobic contributions, but additionally there are close contacts between protein oxygens and positively charged groups in the ligands. The higher affinity of nicotine is due to a main chain hydrogen bond with the B loop and a closer packing of the aromatic groups. These structures will be useful tools for the development of new drugs involving nicotinic acetylcholine receptor-associated diseases.
Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures.,Celie PH, van Rossum-Fikkert SE, van Dijk WJ, Brejc K, Smit AB, Sixma TK Neuron. 2004 Mar 25;41(6):907-14. PMID:15046723[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Celie PH, van Rossum-Fikkert SE, van Dijk WJ, Brejc K, Smit AB, Sixma TK. Nicotine and carbamylcholine binding to nicotinic acetylcholine receptors as studied in AChBP crystal structures. Neuron. 2004 Mar 25;41(6):907-14. PMID:15046723
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