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| | ==THE SOLUTION STRUCTURE OF THE A-FORM OF UROGUANYLIN-16 NMR, 10 STRUCTURES== | | ==THE SOLUTION STRUCTURE OF THE A-FORM OF UROGUANYLIN-16 NMR, 10 STRUCTURES== |
| - | <StructureSection load='1uya' size='340' side='right'caption='[[1uya]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''> | + | <StructureSection load='1uya' size='340' side='right'caption='[[1uya]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1uya]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UYA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1UYA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1uya]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1UYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1UYA FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1uya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uya OCA], [http://pdbe.org/1uya PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1uya RCSB], [http://www.ebi.ac.uk/pdbsum/1uya PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1uya ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 10 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1uya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1uya OCA], [https://pdbe.org/1uya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1uya RCSB], [https://www.ebi.ac.uk/pdbsum/1uya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1uya ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GUC2B_HUMAN GUC2B_HUMAN]] Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport. | + | [https://www.uniprot.org/uniprot/GUC2B_HUMAN GUC2B_HUMAN] Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Adermann, K]] | + | [[Category: Adermann K]] |
| - | [[Category: Forssmann, W G]] | + | [[Category: Forssmann W-G]] |
| - | [[Category: Marx, U C]] | + | [[Category: Marx UC]] |
| - | [[Category: Roesch, P]] | + | [[Category: Roesch P]] |
| - | [[Category: Guanylyl cyclase c activation]]
| + | |
| - | [[Category: Hormone]]
| + | |
| - | [[Category: Isomer some]]
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| - | [[Category: Peptide hormone]]
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| - | [[Category: Solution structure]]
| + | |
| - | [[Category: Topological stereoisomer]]
| + | |
| - | [[Category: Uroguanylin]]
| + | |
| Structural highlights
Function
GUC2B_HUMAN Endogenous activator of intestinal guanylate cyclase. It stimulates this enzyme through the same receptor binding region as the heat-stable enterotoxins. May be a potent physiological regulator of intestinal fluid and electrolyte transport. May be an autocrine/paracrine regulator of intestinal salt and water transport.
Publication Abstract from PubMed
The peptide hormone uroguanylin stimulates chloride secretion via activation of intestinal guanylyl cyclase C (GC-C). It is characterized by two disulfide bonds in a 1-3/2-4 pattern that causes the existence of two topological stereoisomers of which only one induces intracellular cGMP elevation. To obtain an unambiguous structure-function relationship of the isomers, we determined the solution structure of the separated uroguanylin isoforms using NMR spectroscopy. Both isomers adopt well-defined structures that correspond to those of the isomers of the related peptide guanylin. Furthermore, the structure of the GC-C-activating uroguanylin isomer A closely resembles the structure of the agonistic Escherichia coli heat-stable enterotoxin. Compared with guanylin isomers, the conformational interconversion of uroguanylin isomers is retarded significantly. As judged from chromatography and NMR spectroscopy, both uroguanylin isoforms are stable at low temperatures, but are subject to a slow pH-dependent mutual isomerization at 37 degrees C with an equilibrium isomer ratio of approximately 1:1. The conformational exchange is most likely under the sterical control of the carboxy-terminal leucine. These results imply that GC-C is activated by ligands exhibiting the molecular framework corresponding to the structure of uroguanylin isomer A.
One peptide, two topologies: structure and interconversion dynamics of human uroguanylin isomers.,Marx UC, Klodt J, Meyer M, Gerlach H, Rosch P, Forssmann WG, Adermann K J Pept Res. 1998 Sep;52(3):229-40. PMID:9774236[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Marx UC, Klodt J, Meyer M, Gerlach H, Rosch P, Forssmann WG, Adermann K. One peptide, two topologies: structure and interconversion dynamics of human uroguanylin isomers. J Pept Res. 1998 Sep;52(3):229-40. PMID:9774236
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