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| | <StructureSection load='2uwj' size='340' side='right'caption='[[2uwj]], [[Resolution|resolution]] 2.00Å' scene=''> | | <StructureSection load='2uwj' size='340' side='right'caption='[[2uwj]], [[Resolution|resolution]] 2.00Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2uwj]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UWJ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2uwj]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_CHA Pseudomonas aeruginosa CHA]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UWJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UWJ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwj OCA], [http://pdbe.org/2uwj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uwj RCSB], [http://www.ebi.ac.uk/pdbsum/2uwj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwj ProSAT]</span></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uwj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uwj OCA], [https://pdbe.org/2uwj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uwj RCSB], [https://www.ebi.ac.uk/pdbsum/2uwj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uwj ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PSCG_PSEAE PSCG_PSEAE]] Prevents premature polymerization of PscF, along with PscE, within the cytoplasm. Required for type III secretion needle assembly. Also required for cytotoxicity by influencing PscF levels. | + | [https://www.uniprot.org/uniprot/PSCF_PSEAE PSCF_PSEAE] Major component of the type III secretion needle structure. Required for cytotoxicity on macrophages.<ref>PMID:16239085</ref> <ref>PMID:16115870</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Attree, I]] | + | [[Category: Pseudomonas aeruginosa CHA]] |
| - | [[Category: Contreras-Martel, C]] | + | [[Category: Attree I]] |
| - | [[Category: Dessen, A]] | + | [[Category: Contreras-Martel C]] |
| - | [[Category: Job, V]] | + | [[Category: Dessen A]] |
| - | [[Category: Ple, S]] | + | [[Category: Job V]] |
| - | [[Category: Quinaud, M]] | + | [[Category: Ple S]] |
| - | [[Category: Simorre, J P]] | + | [[Category: Quinaud M]] |
| - | [[Category: Bacterial pathogenicity]]
| + | [[Category: Simorre JP]] |
| - | [[Category: Chaperone]]
| + | |
| - | [[Category: Coiled coil]]
| + | |
| - | [[Category: Needle formation]]
| + | |
| - | [[Category: Type iii secretion]]
| + | |
| - | [[Category: Virulence]]
| + | |
| Structural highlights
Function
PSCF_PSEAE Major component of the type III secretion needle structure. Required for cytotoxicity on macrophages.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Type III secretion systems (T3SS), found in several Gram-negative pathogens, are nanomachines involved in the transport of virulence effectors directly into the cytoplasm of target cells. T3SS are essentially composed of basal membrane-embedded ring-like structures and a hollow needle formed by a single polymerized protein. Within the bacterial cytoplasm, the T3SS needle protein requires two distinct chaperones for stabilization before its secretion, without which the entire T3SS is nonfunctional. The 2.0-A x-ray crystal structure of the PscE-PscF(55-85)-PscG heterotrimeric complex from Pseudomonas aeruginosa reveals that the C terminus of the needle protein PscF is engulfed within the hydrophobic groove of the tetratricopeptide-like molecule PscG, indicating that the macromolecular scaffold necessary to stabilize the T3SS needle is totally distinct from chaperoned complexes between pilus- or flagellum-forming molecules. Disruption of specific PscG-PscF interactions leads to impairment of bacterial cytotoxicity toward macrophages, indicating that this essential heterotrimer, which possesses homologs in a wide variety of pathogens, is a unique attractive target for the development of novel antibacterials.
Structure of the heterotrimeric complex that regulates type III secretion needle formation.,Quinaud M, Ple S, Job V, Contreras-Martel C, Simorre JP, Attree I, Dessen A Proc Natl Acad Sci U S A. 2007 May 8;104(19):7803-8. Epub 2007 Apr 30. PMID:17470796[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pastor A, Chabert J, Louwagie M, Garin J, Attree I. PscF is a major component of the Pseudomonas aeruginosa type III secretion needle. FEMS Microbiol Lett. 2005 Dec 1;253(1):95-101. Epub 2005 Oct 5. PMID:16239085 doi:http://dx.doi.org/S0378-1097(05)00665-8
- ↑ Quinaud M, Chabert J, Faudry E, Neumann E, Lemaire D, Pastor A, Elsen S, Dessen A, Attree I. The PscE-PscF-PscG complex controls type III secretion needle biogenesis in Pseudomonas aeruginosa. J Biol Chem. 2005 Oct 28;280(43):36293-300. Epub 2005 Aug 22. PMID:16115870 doi:M508089200
- ↑ Quinaud M, Ple S, Job V, Contreras-Martel C, Simorre JP, Attree I, Dessen A. Structure of the heterotrimeric complex that regulates type III secretion needle formation. Proc Natl Acad Sci U S A. 2007 May 8;104(19):7803-8. Epub 2007 Apr 30. PMID:17470796 doi:0610098104
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