6ttj
From Proteopedia
(Difference between revisions)
(New page: '''Unreleased structure''' The entry 6ttj is ON HOLD Authors: Tsirkone, V.G., Osipov, E.M., Beelen, S., Strelkov, S.V. Description: Neutral invertase 2 from Arabidopsis thaliana [[Cate...) |
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| - | '''Unreleased structure''' | ||
| - | + | ==Neutral invertase 2 from Arabidopsis thaliana== | |
| + | <StructureSection load='6ttj' size='340' side='right'caption='[[6ttj]], [[Resolution|resolution]] 3.39Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6ttj]] is a 12 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TTJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TTJ FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.392Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ttj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ttj OCA], [https://pdbe.org/6ttj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ttj RCSB], [https://www.ebi.ac.uk/pdbsum/6ttj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ttj ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/CINV1_ARATH CINV1_ARATH] Cytosolic invertase that specifically cleaves sucrose into glucose and fructose and is involved in the regulation of multiple tissue development including primary root elongation, root hair growth, leaf and silique development, and floral transition. Is involved in osmotic stress-induced inhibition on lateral root growth by controlling the concentration of hexose in cells. May regulate sugar-mediated root development by controlling sucrose catabolism in root cells.<ref>PMID:17220200</ref> <ref>PMID:17508130</ref> <ref>PMID:19470642</ref> <ref>PMID:21441406</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The metabolism of sucrose is of crucial importance for life on Earth. In plants, enzymes called invertases split sucrose into glucose and fructose, contributing to the regulation of metabolic fluxes. Invertases differ in their localization and pH optimum. Acidic invertases present in plant cell walls and vacuoles belong to glycoside hydrolase family 32 (GH32) and have an all-beta structure. In contrast, neutral invertases are located in the cytosol and organelles such as chloroplasts and mitochondria. These poorly understood enzymes are classified into a separate GH100 family. Recent crystal structures of the closely related neutral invertases InvA and InvB from the cyanobacterium Anabaena revealed a predominantly alpha-helical fold with unique features compared with other sucrose-metabolizing enzymes. Here, a neutral invertase (AtNIN2) from the model plant Arabidopsis thaliana was heterologously expressed, purified and crystallized. As a result, the first neutral invertase structure from a higher plant has been obtained at 3.4 A resolution. The hexameric AtNIN2 structure is highly similar to that of InvA, pointing to high evolutionary conservation of neutral invertases. | ||
| - | + | Crystal structure of Arabidopsis thaliana neutral invertase 2.,Tarkowski LP, Tsirkone VG, Osipov EM, Beelen S, Lammens W, Vergauwen R, Van den Ende W, Strelkov SV Acta Crystallogr F Struct Biol Commun. 2020 Mar 1;76(Pt 3):152-157. doi:, 10.1107/S2053230X2000179X. Epub 2020 Mar 3. PMID:32134001<ref>PMID:32134001</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 6ttj" style="background-color:#fffaf0;"></div> |
| - | [[Category: Osipov | + | |
| - | [[Category: | + | ==See Also== |
| - | [[Category: | + | *[[Invertase|Invertase]] |
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Arabidopsis thaliana]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Beelen S]] | ||
| + | [[Category: Osipov EM]] | ||
| + | [[Category: Strelkov SV]] | ||
| + | [[Category: Tsirkone VG]] | ||
Current revision
Neutral invertase 2 from Arabidopsis thaliana
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