6sjb

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==Cryo-EM structure of the RecBCD Chi recognised complex==
==Cryo-EM structure of the RecBCD Chi recognised complex==
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<StructureSection load='6sjb' size='340' side='right'caption='[[6sjb]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
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<SX load='6sjb' size='340' side='right' viewer='molstar' caption='[[6sjb]], [[Resolution|resolution]] 3.70&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[6sjb]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SJB FirstGlance]. <br>
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<table><tr><td colspan='2'>[[6sjb]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SJB FirstGlance]. <br>
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</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">recB_1, recB, AUQ13_17740, BANRA_01341, BANRA_04284, BHS81_16925, BUE81_06765, BvCms2454_02089, BvCmsHHP001_01116, BvCmsHHP056_02020, BvCmsNSNP006_02180, BvCmsNSNP027_02943, BvCmsSINP011_00475, BvCmsSIP044_01651, C4J69_11455, C5N07_06355, D9I18_10890, DNQ41_19170, E5M00_06020, EAI44_21005, EAI52_16505, EC3234A_49c00050, ECTO6_01026, EFV07_08645, EPS71_15035, ERL57_14525, EXX32_11785, EXX39_20385, EXX71_16045, EYD11_05005, NCTC11022_02903, NCTC13462_04762, NCTC9117_01550, NCTC9706_03254, RG28_07470, SAMEA3472080_03114, SAMEA3752559_02953, SAMEA3753300_01149 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), recC, b2822, JW2790 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895]), recD, recD_2, ACU57_24225, AM464_05810, AUQ13_17735, BHS81_16920, BUE81_06770, BvCms2454_02088, BvCmsHHP001_01117, BvCmsHHP056_02019, BvCmsKSP036_04400, BvCmsNSNP006_02181, BvCmsNSNP027_02942, BvCmsSINP011_00476, BvCmsSINP012_00057, BvCmsSIP044_01650, BVL39_12765, C4J69_11460, C5N07_06350, C7B02_19600, CG692_06815, CRM83_26155, D3821_10935, D9G42_05835, D9H94_17780, D9I18_10885, D9K48_23640, DNQ41_19165, E4Z89_04655, EAI44_21010, EC3234A_49c00040, ECTO6_01027, EEP23_14080, EFB45_23490, EL75_0877, EL79_0879, EL80_0882, ERL57_14520, EXX24_12005, EXX32_11780, EXX71_16050, EYD11_05010, EYY78_03865, HW43_18500, NCTC11022_02902, NCTC13148_02729, NCTC8500_01037, NCTC9036_01094, NCTC9037_01202, NCTC9062_02173, NCTC9117_01551, NCTC9706_03255, RG28_07475, RK56_023370, SAMEA3472047_00298, SAMEA3472080_03115, SAMEA3484427_03257, SAMEA3484429_03374, SAMEA3752559_02954, SAMEA3753300_01148, SK85_03061 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.7&#8491;</td></tr>
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<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Exodeoxyribonuclease_V Exodeoxyribonuclease V], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.11.5 3.1.11.5] </span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sjb OCA], [https://pdbe.org/6sjb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sjb RCSB], [https://www.ebi.ac.uk/pdbsum/6sjb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sjb ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sjb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sjb OCA], [http://pdbe.org/6sjb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sjb RCSB], [http://www.ebi.ac.uk/pdbsum/6sjb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sjb ProSAT]</span></td></tr>
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</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/A0A024LB08_ECOLX A0A024LB08_ECOLX]] A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA.[HAMAP-Rule:MF_01485] [[http://www.uniprot.org/uniprot/A0A061K747_ECOLX A0A061K747_ECOLX]] A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a highly rapid and processive ATP-dependent bidirectional helicase activity. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator) sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to the Chi site. The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang and facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination. In the holoenzyme this subunit has ssDNA-dependent ATPase and 5'-3' helicase activity. When added to pre-assembled RecBC greatly stimulates nuclease activity and augments holoenzyme processivity. Negatively regulates the RecA-loading ability of RecBCD.[HAMAP-Rule:MF_01487] [[http://www.uniprot.org/uniprot/RECC_ECOLI RECC_ECOLI]] A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid (>1 kb/second) and highly processive (>30 kb) ATP-dependent bidirectional helicase. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator, 5'-GCTGGTGG-3') sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to Chi site, by nicking one strand or switching the strand degraded (depending on the reaction conditions). The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang which facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination (PubMed:4562392, PubMed:4552016, PubMed:123277). In the holoenzyme this subunit almost certainly recognizes the wild-type Chi sequence, when added to isolated RecB increases its ATP-dependent helicase processivity. The RecBC complex requires the RecD subunit for nuclease activity, but can translocate along ssDNA in both directions.<ref>PMID:10197988</ref> <ref>PMID:10884344</ref> <ref>PMID:123277</ref> <ref>PMID:12815437</ref> <ref>PMID:12815438</ref> <ref>PMID:1535156</ref> <ref>PMID:16041061</ref> <ref>PMID:1618858</ref> <ref>PMID:20852646</ref> <ref>PMID:23851395</ref> <ref>PMID:4268693</ref> <ref>PMID:4552016</ref> <ref>PMID:4562392</ref> <ref>PMID:7608206</ref> <ref>PMID:9192629</ref> <ref>PMID:9230304</ref> <ref>PMID:9448271</ref> <ref>PMID:9790841</ref>
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[https://www.uniprot.org/uniprot/RECB_ECOLI RECB_ECOLI] A helicase/nuclease that prepares dsDNA breaks (DSB) for recombinational DNA repair. Binds to DSBs and unwinds DNA via a rapid (>1 kb/second) and highly processive (>30 kb) ATP-dependent bidirectional helicase. Unwinds dsDNA until it encounters a Chi (crossover hotspot instigator, 5'-GCTGGTGG-3') sequence from the 3' direction. Cuts ssDNA a few nucleotides 3' to Chi site, by nicking one strand or switching the strand degraded (depending on the reaction conditions). The properties and activities of the enzyme are changed at Chi. The Chi-altered holoenzyme produces a long 3'-ssDNA overhang which facilitates RecA-binding to the ssDNA for homologous DNA recombination and repair. Holoenzyme degrades any linearized DNA that is unable to undergo homologous recombination (PubMed:4562392, PubMed:4552016, PubMed:123277). In the holoenzyme this subunit contributes ATPase, 3'-5' helicase, exonuclease activity and loads RecA onto ssDNA. The RecBC complex requires the RecD subunit for nuclease activity, but can translocate along ssDNA in both directions.<ref>PMID:10197988</ref> <ref>PMID:10518611</ref> <ref>PMID:10766864</ref> <ref>PMID:123277</ref> <ref>PMID:12815437</ref> <ref>PMID:12815438</ref> <ref>PMID:1535156</ref> <ref>PMID:16041061</ref> <ref>PMID:1618858</ref> <ref>PMID:16388588</ref> <ref>PMID:18079176</ref> <ref>PMID:20852646</ref> <ref>PMID:23851395</ref> <ref>PMID:25073102</ref> <ref>PMID:4268693</ref> <ref>PMID:4552016</ref> <ref>PMID:4562392</ref> <ref>PMID:7608206</ref> <ref>PMID:9192629</ref> <ref>PMID:9230304</ref> <ref>PMID:9448271</ref> <ref>PMID:9790841</ref>
<div style="background-color:#fffaf0;">
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
== Publication Abstract from PubMed ==
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</div>
</div>
<div class="pdbe-citations 6sjb" style="background-color:#fffaf0;"></div>
<div class="pdbe-citations 6sjb" style="background-color:#fffaf0;"></div>
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==See Also==
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*[[Exonuclease 3D structures|Exonuclease 3D structures]]
== References ==
== References ==
<references/>
<references/>
__TOC__
__TOC__
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</StructureSection>
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</SX>
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[[Category: Bacillus coli migula 1895]]
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[[Category: Escherichia coli]]
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[[Category: Exodeoxyribonuclease V]]
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[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Cheng, K]]
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[[Category: Synthetic construct]]
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[[Category: Wigley, D B]]
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[[Category: Cheng K]]
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[[Category: Wilkinson, M]]
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[[Category: Wigley DB]]
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[[Category: Atp hydrolysis]]
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[[Category: Wilkinson M]]
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[[Category: Dna repair]]
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[[Category: Helicase]]
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[[Category: Homologous recombination]]
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[[Category: Hydrolase]]
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[[Category: Nuclease]]
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[[Category: Translocation]]
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Current revision

Cryo-EM structure of the RecBCD Chi recognised complex

6sjb, resolution 3.70Å

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