1elt

From Proteopedia

(Difference between revisions)

Current revision

STRUCTURE OF NATIVE PANCREATIC ELASTASE FROM NORTH ATLANTIC SALMON AT 1.61 ANGSTROMS RESOLUTION

Structural highlights

1elt is a 1 chain structure with sequence from Salmo salar. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.61Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

ELA1_SALSA Acts upon elastin.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The crystal structure of native salmon pancreatic elastase (SPE) has been solved by molecular-replacement methods, and refined by conventional conjugate-gradient methods and simulated-annealing techniques. The final R value is 17.2% for 21 389 reflections between 8.0 and 1.61 A, and the corresponding free R value is 23.9%. The overall tertiary structure of SPE is remarkably similar to that of porcine pancreatic elastase I (PPE), to which it shows about 67% sequence identity. The primary structure of SPE is determined from the electron-density maps, and only about 15 side chains are somewhat uncertain. Interesting differences between SPE and PPE, are one sequence deletion assigned to position 186, the residue 192 at the entrance of the specificity pocket is substituted from a Gln in PPE to Asn in SPE, and one of the calcium ligands is different. Furthermore, electron density is missing in SPE for the last three residues of the C-terminal helix. A comparison of the present amino-acid sequence of SPE with other sequences available indicates that SPE belongs to the class 1 pancreatic elastases.

Structure of native pancreatic elastase from North Atlantic salmon at 1.61 A resolution.,Berglund GI, Willassen NP, Hordvik A, Smalas AO Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):925-37. PMID:15299762^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Berglund GI, Smalas AO, Outzen H, Willassen NP. Purification and characterization of pancreatic elastase from North Atlantic salmon (Salmo salar). Mol Mar Biol Biotechnol. 1998 Jun;7(2):105-14. PMID:9628006
↑ Berglund GI, Willassen NP, Hordvik A, Smalas AO. Structure of native pancreatic elastase from North Atlantic salmon at 1.61 A resolution. Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):925-37. PMID:15299762 doi:10.1107/S0907444995004835

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1elt"

Categories: Large Structures | Salmo salar | Berglund GI | Smalaas AO

@@ Line 3: / Line 3: @@
 <StructureSection load='1elt' size='340' side='right'caption='[[1elt]], [[Resolution|resolution]] 1.61&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1elt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ELT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1elt]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmo_salar Salmo salar]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ELT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ELT FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.61&#8491;</td></tr>
-<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_elastase Pancreatic elastase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.36 3.4.21.36] </span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1elt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1elt OCA], [http://pdbe.org/1elt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1elt RCSB], [http://www.ebi.ac.uk/pdbsum/1elt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1elt ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1elt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1elt OCA], [https://pdbe.org/1elt PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1elt RCSB], [https://www.ebi.ac.uk/pdbsum/1elt PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1elt ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/ELA1_SALSA ELA1_SALSA]] Acts upon elastin.<ref>PMID:9628006</ref>
+[https://www.uniprot.org/uniprot/ELA1_SALSA ELA1_SALSA] Acts upon elastin.<ref>PMID:9628006</ref>
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 15: / Line 15: @@
   <jmolCheckbox>
     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/el/1elt_consurf.spt"</scriptWhenChecked>
-    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
@@ Line 37: / Line 37: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Pancreatic elastase]]
 [[Category: Salmo salar]]
-[[Category: Berglund, G I]]
+[[Category: Berglund GI]]
-[[Category: Smalaas, A O]]
+[[Category: Smalaas AO]]
-[[Category: Serine proteinase]]

1elt

From Proteopedia

Current revision

STRUCTURE OF NATIVE PANCREATIC ELASTASE FROM NORTH ATLANTIC SALMON AT 1.61 ANGSTROMS RESOLUTION

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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