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| | <StructureSection load='1xj9' size='340' side='right'caption='[[1xj9]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='1xj9' size='340' side='right'caption='[[1xj9]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1xj9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJ9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XJ9 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1xj9]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XJ9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XJ9 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=APN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-ADENINE'>APN</scene>, <scene name='pdbligand=CPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-CYTOSINE'>CPN</scene>, <scene name='pdbligand=GPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-GUANINE'>GPN</scene>, <scene name='pdbligand=TPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-THYMINE'>TPN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1pup|1pup]], [[1pdt|1pdt]], [[1pnn|1pnn]], [[176d|176d]], [[1nr8|1nr8]], [[1rru|1rru]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=APN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-ADENINE'>APN</scene>, <scene name='pdbligand=CPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-CYTOSINE'>CPN</scene>, <scene name='pdbligand=GPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-GUANINE'>GPN</scene>, <scene name='pdbligand=TPN:2-AMINOETHYLGLYCINE-CARBONYLMETHYLENE-THYMINE'>TPN</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xj9 OCA], [http://pdbe.org/1xj9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xj9 RCSB], [http://www.ebi.ac.uk/pdbsum/1xj9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xj9 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xj9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xj9 OCA], [https://pdbe.org/1xj9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xj9 RCSB], [https://www.ebi.ac.uk/pdbsum/1xj9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xj9 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gajhede, M]] | + | [[Category: Gajhede M]] |
| - | [[Category: Kastrup, J S]] | + | [[Category: Kastrup JS]] |
| - | [[Category: Larsen, I K]] | + | [[Category: Larsen IK]] |
| - | [[Category: Nielsen, B B]] | + | [[Category: Nielsen BB]] |
| - | [[Category: Nielsen, P E]] | + | [[Category: Nielsen PE]] |
| - | [[Category: Petersson, B]] | + | [[Category: Petersson B]] |
| - | [[Category: Rasmussen, H]] | + | [[Category: Rasmussen H]] |
| - | [[Category: Duplex-triplex complex]]
| + | |
| - | [[Category: Left-handed]]
| + | |
| - | [[Category: Partly self-complementary]]
| + | |
| - | [[Category: Peptide nucleic acid]]
| + | |
| - | [[Category: Pna]]
| + | |
| - | [[Category: Right-handed]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The X-ray structure of a partly self-complementary peptide nucleic acid (PNA) decamer (H-GTAGATCACT-l-Lys-NH(2)) to 2.60 A resolution is reported. The structure is mainly controlled by the canonical Watson-Crick base pairs formed by the self-complementary stretch of four bases in the middle of the decamer (G(4)A(5)T(6)C(7)). One right- and one left-handed Watson-Crick duplex are formed. The two PNA units C(9)T(10) change helical handedness, so that each PNA strand contains both a right- and a left-handed section. The changed handedness in C(9)T(10) allows formation of Hoogsteen hydrogen bonding between C(9)T(10) and G(4)A(5) of a PNA strand in an adjacent Watson-Crick double helix of the same handedness. Thereby, a PNA-PNA-PNA triplex is formed. The PNA unit A(3) forms a noncanonical base pair with A(8) in a symmetry-related strand of opposite handedness; the base pair is of the A-A reverse Hoogsteen type. The structural diversity of this PNA demonstrates how the PNA backbone is able to adapt to structures governed by the stacking and hydrogen-bonding interactions between the nucleobases. The crystal structure further shows how PNA oligomers containing limited sequence complementarity may form complex hydrogen-bonding networks.
Crystal structure of a partly self-complementary peptide nucleic acid (PNA) oligomer showing a duplex-triplex network.,Petersson B, Nielsen BB, Rasmussen H, Larsen IK, Gajhede M, Nielsen PE, Kastrup JS J Am Chem Soc. 2005 Feb 9;127(5):1424-30. PMID:15686374[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Petersson B, Nielsen BB, Rasmussen H, Larsen IK, Gajhede M, Nielsen PE, Kastrup JS. Crystal structure of a partly self-complementary peptide nucleic acid (PNA) oligomer showing a duplex-triplex network. J Am Chem Soc. 2005 Feb 9;127(5):1424-30. PMID:15686374 doi:10.1021/ja0458726
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