This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1u0d

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
(New page: 200px<br /> <applet load="1u0d" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u0d, resolution 2.9&Aring;" /> '''Y33H Mutatant of Hom...)
Current revision (08:43, 14 February 2024) (edit) (undo)
 
(17 intermediate revisions not shown.)
Line 1: Line 1:
-
[[Image:1u0d.gif|left|200px]]<br />
 
-
<applet load="1u0d" size="450" color="white" frame="true" align="right" spinBox="true"
 
-
caption="1u0d, resolution 2.9&Aring;" />
 
-
'''Y33H Mutatant of Homing endonuclease I-CreI'''<br />
 
-
==Overview==
+
==Y33H Mutant of Homing endonuclease I-CreI==
-
Homing endonucleases are highly specific DNA endonucleases, encoded within, mobile introns or inteins, that induce targeted recombination, double-strand repair and gene conversion of their cognate target sites., Due to their biological function and high level of target specificity, these enzymes are under intense investigation as tools for gene targeting., These studies require that naturally occurring enzymes be redesigned to, recognize novel target sites. Here, we report studies in which the, homodimeric LAGLIDADG homing endonuclease I-CreI is altered at individual, side-chains corresponding to contact points to distinct base-pairs in its, target site. The resulting enzyme constructs drive specific elimination of, selected DNA targets in vivo and display shifted specificities of DNA, binding and cleavage in vitro. Crystal structures of two of these, constructs demonstrate that substitution of individual side-chain/DNA, contact patterns can occur with almost no structural deformation or, rearrangement of the surrounding complex, facilitating an isolated, modular redesign strategy for homing endonuclease activity and, specificity.
+
<StructureSection load='1u0d' size='340' side='right'caption='[[1u0d]], [[Resolution|resolution]] 2.90&Aring;' scene=''>
 +
== Structural highlights ==
 +
<table><tr><td colspan='2'>[[1u0d]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1U0D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1U0D FirstGlance]. <br>
 +
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9&#8491;</td></tr>
 +
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1u0d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u0d OCA], [https://pdbe.org/1u0d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1u0d RCSB], [https://www.ebi.ac.uk/pdbsum/1u0d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1u0d ProSAT]</span></td></tr>
 +
</table>
 +
== Function ==
 +
[https://www.uniprot.org/uniprot/DNE1_CHLRE DNE1_CHLRE] Endonuclease involved in group I intron homing. Recognizes and cleaves a 19-24 bp palindromic DNA site.
 +
== Evolutionary Conservation ==
 +
[[Image:Consurf_key_small.gif|200px|right]]
 +
Check<jmol>
 +
<jmolCheckbox>
 +
<scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u0d_consurf.spt"</scriptWhenChecked>
 +
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 +
<text>to colour the structure by Evolutionary Conservation</text>
 +
</jmolCheckbox>
 +
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1u0d ConSurf].
 +
<div style="clear:both"></div>
-
==About this Structure==
+
==See Also==
-
1U0D is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Chlamydomonas_reinhardtii Chlamydomonas reinhardtii]. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1U0D OCA].
+
*[[Endonuclease 3D structures|Endonuclease 3D structures]]
-
 
+
__TOC__
-
==Reference==
+
</StructureSection>
-
Isolation and characterization of new homing endonuclease specificities at individual target site positions., Sussman D, Chadsey M, Fauce S, Engel A, Bruett A, Monnat R Jr, Stoddard BL, Seligman LM, J Mol Biol. 2004 Sep 3;342(1):31-41. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=15313605 15313605]
+
[[Category: Chlamydomonas reinhardtii]]
[[Category: Chlamydomonas reinhardtii]]
-
[[Category: Single protein]]
+
[[Category: Large Structures]]
-
[[Category: Bruett, A.]]
+
[[Category: Bruett A]]
-
[[Category: Chadsey, M.]]
+
[[Category: Chadsey M]]
-
[[Category: Engel, A.]]
+
[[Category: Engel A]]
-
[[Category: Fauce, S.]]
+
[[Category: Fauce S]]
-
[[Category: Monnat, R.]]
+
[[Category: Monnat R]]
-
[[Category: Seligman, L.M.]]
+
[[Category: Seligman LM]]
-
[[Category: Stoddard, B.L.]]
+
[[Category: Stoddard BL]]
-
[[Category: Sussman, D.]]
+
[[Category: Sussman D]]
-
[[Category: dna endonuclease i-crei]]
+
-
[[Category: protein/dna]]
+
-
 
+
-
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Thu Nov 8 12:44:18 2007''
+

Current revision

Y33H Mutant of Homing endonuclease I-CreI

PDB ID 1u0d

Drag the structure with the mouse to rotate

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1u0d"
Personal tools