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| | <StructureSection load='6sd0' size='340' side='right'caption='[[6sd0]], [[Resolution|resolution]] 3.70Å' scene=''> | | <StructureSection load='6sd0' size='340' side='right'caption='[[6sd0]], [[Resolution|resolution]] 3.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6sd0]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thema Thema]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SD0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SD0 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sd0]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SD0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SD0 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lacZ, TM_1193 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=243274 THEMA])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-galactosidase Beta-galactosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.23 3.2.1.23] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sd0 OCA], [https://pdbe.org/6sd0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sd0 RCSB], [https://www.ebi.ac.uk/pdbsum/6sd0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sd0 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sd0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sd0 OCA], [http://pdbe.org/6sd0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sd0 RCSB], [http://www.ebi.ac.uk/pdbsum/6sd0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sd0 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/BGAL_THEMA BGAL_THEMA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6sd0" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6sd0" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Galactosidase 3D structures|Galactosidase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Beta-galactosidase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thema]] | + | [[Category: Thermotoga maritima MSB8]] |
| - | [[Category: Jimenez-Ortega, E]] | + | [[Category: Jimenez-Ortega E]] |
| - | [[Category: Ramirez-Escudero, M]] | + | [[Category: Ramirez-Escudero M]] |
| - | [[Category: Sanz-Aparicio, J]] | + | [[Category: Sanz-Aparicio J]] |
| - | [[Category: Galactooligosaccharide]]
| + | |
| - | [[Category: Glycoside hydrolase]]
| + | |
| - | [[Category: Glycosyl hydrolase 2 family. sugar binding protein]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Hydrolysis]]
| + | |
| - | [[Category: Lactose]]
| + | |
| - | [[Category: Thermoresistant]]
| + | |
| - | [[Category: Thermotoga maritima]]
| + | |
| Structural highlights
Function
BGAL_THEMA
Publication Abstract from PubMed
Lactose intolerance is a common digestive disorder that affects a large proportion of the adult human population. The severity of the symptoms is highly variable, depending on the susceptibility to the sugar and the amount digested. For that reason, enzymes that can be used for the production of lactose-free milk and milk derivatives have acquired singular biotechnological importance. One such case is Thermotoga maritima beta-galactosidase (TmLac). Here, we report the cryo-EM structure of TmLac at 2.0 A resolution. The protein features a newly solved domain at its C-terminus, characteristic of the genus Thermotoga, which promotes a peculiar octameric arrangement. We have assessed the constraints imposed by the quaternary protein structure on the construction of hybrid versions of this GH2 enzyme. Carbohydrate binding modules (CBM) from the CBM2 and CBM9 families have been added at either the amino or carboxy terminus, and the structural and functional effects of such modifications have been analyzed. The results provide a basis for the rational design of hybrid enzymes that can be efficiently attached to different solid supports.
The cryo-EM Structure of Thermotoga maritima beta-Galactosidase: Quaternary Structure Guides Protein Engineering.,Miguez Amil S, Jimenez-Ortega E, Ramirez-Escudero M, Talens-Perales D, Marin-Navarro J, Polaina J, Sanz-Aparicio J, Fernandez-Leiro R ACS Chem Biol. 2020 Jan 17;15(1):179-188. doi: 10.1021/acschembio.9b00752. Epub, 2019 Dec 24. PMID:31874027[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Miguez Amil S, Jimenez-Ortega E, Ramirez-Escudero M, Talens-Perales D, Marin-Navarro J, Polaina J, Sanz-Aparicio J, Fernandez-Leiro R. The cryo-EM Structure of Thermotoga maritima beta-Galactosidase: Quaternary Structure Guides Protein Engineering. ACS Chem Biol. 2020 Jan 17;15(1):179-188. doi: 10.1021/acschembio.9b00752. Epub, 2019 Dec 24. PMID:31874027 doi:http://dx.doi.org/10.1021/acschembio.9b00752
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