|
|
| (One intermediate revision not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Solution Structure of the ScYLV P1-P2 Frameshifting Pseudoknot, Regularized Average Structure== | | ==Solution Structure of the ScYLV P1-P2 Frameshifting Pseudoknot, Regularized Average Structure== |
| - | <StructureSection load='1yg4' size='340' side='right'caption='[[1yg4]], [[NMR_Ensembles_of_Models | 1 NMR models]]' scene=''> | + | <StructureSection load='1yg4' size='340' side='right'caption='[[1yg4]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1yg4]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YG4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YG4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1yg4]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YG4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YG4 FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CH:N3-PROTONATED+CYTIDINE-5-MONOPHOSPHATE'>CH</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1yg3|1yg3]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CH:N3-PROTONATED+CYTIDINE-5-MONOPHOSPHATE'>CH</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yg4 OCA], [http://pdbe.org/1yg4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yg4 RCSB], [http://www.ebi.ac.uk/pdbsum/1yg4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yg4 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yg4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yg4 OCA], [https://pdbe.org/1yg4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yg4 RCSB], [https://www.ebi.ac.uk/pdbsum/1yg4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yg4 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| Line 22: |
Line 22: |
| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Cornish, P V]] | + | [[Category: Cornish PV]] |
| - | [[Category: Giedroc, D P]] | + | [[Category: Giedroc DP]] |
| - | [[Category: Hennig, M]] | + | [[Category: Hennig M]] |
| - | [[Category: Protonated cytidine]]
| + | |
| - | [[Category: Ribosomal frameshifting]]
| + | |
| - | [[Category: Rna]]
| + | |
| - | [[Category: Rna pseudoknot]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The molecular determinants of stimulation of -1 programmed ribosomal frameshifting (-1 PRF) by RNA pseudoknots are poorly understood. Sugarcane yellow leaf virus (ScYLV) encodes a 28-nt mRNA pseudoknot that promotes -1 PRF between the P1 (protease) and P2 (polymerase) genes in plant luteoviruses. The solution structure of the ScYLV pseudoknot reveals a well ordered loop 2 (L2) that exhibits continuous stacking of A20 through C27 in the minor groove of the upper stem 1 (S1), with C25 flipped out of the triple-stranded stack. Five consecutive triple base pairs flank the helical junction where the 3' nucleotide of L2, C27, adopts a cytidine 27 N3-cytidine 14 2'-OH hydrogen bonding interaction with the C14-G7 base pair. This interaction is isosteric with the adenosine N1-2'-OH interaction in the related mRNA from beet western yellows virus (BWYV); however, the ScYLV and BWYV mRNA structures differ in their detailed L2-S1 hydrogen bonding and L2 stacking interactions. Functional analyses of ScYLV/BWYV chimeric pseudoknots reveal that the ScYLV RNA stimulates a higher level of -1 PRF (15 +/- 2%) relative to the BWYV pseudoknot (6 +/- 1%), a difference traced largely to the identity of the 3' nucleotide of L2 (C27 vs. A25 in BWYV). Strikingly, C27A ScYLV RNA is a poor frameshift stimulator (2.0%) and is destabilized by approximately 1.5 kcal x mol(-1) (pH 7.0, 37 degrees C) with respect to the wild-type pseudoknot. These studies establish that the precise network of weak interactions nearest the helical junction in structurally similar pseudoknots make an important contribution to setting the frameshift efficiency in mRNAs.
A loop 2 cytidine-stem 1 minor groove interaction as a positive determinant for pseudoknot-stimulated -1 ribosomal frameshifting.,Cornish PV, Hennig M, Giedroc DP Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12694-9. Epub 2005 Aug 25. PMID:16123125[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cornish PV, Hennig M, Giedroc DP. A loop 2 cytidine-stem 1 minor groove interaction as a positive determinant for pseudoknot-stimulated -1 ribosomal frameshifting. Proc Natl Acad Sci U S A. 2005 Sep 6;102(36):12694-9. Epub 2005 Aug 25. PMID:16123125 doi:0506166102
|
