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| | ==High-resolution solution structure of the LM5-1 FYVE domain from Leishmania major== | | ==High-resolution solution structure of the LM5-1 FYVE domain from Leishmania major== |
| - | <StructureSection load='1z2q' size='340' side='right'caption='[[1z2q]], [[NMR_Ensembles_of_Models | 21 NMR models]]' scene=''> | + | <StructureSection load='1z2q' size='340' side='right'caption='[[1z2q]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1z2q]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leima Leima]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2Q OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z2Q FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1z2q]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z2Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1Z2Q FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z2q OCA], [http://pdbe.org/1z2q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z2q RCSB], [http://www.ebi.ac.uk/pdbsum/1z2q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z2q ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1z2q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z2q OCA], [https://pdbe.org/1z2q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1z2q RCSB], [https://www.ebi.ac.uk/pdbsum/1z2q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1z2q ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Leima]] | + | [[Category: Leishmania major]] |
| - | [[Category: Callaghan, J M]] | + | [[Category: Callaghan JM]] |
| - | [[Category: Gooley, P R]] | + | [[Category: Gooley PR]] |
| - | [[Category: McConville, M J]] | + | [[Category: McConville MJ]] |
| - | [[Category: Mertens, H D.T]] | + | [[Category: Mertens HDT]] |
| - | [[Category: Fyve domain]]
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| - | [[Category: Membrane protein]]
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| - | [[Category: Zinc-finger]]
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| Structural highlights
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
FYVE domain proteins play key roles in regulating membrane traffic in eukaryotic cells. The FYVE domain displays a remarkable specificity for the head group of the target lipid, phosphatidylinositol 3-phosphate (PtdIns[3]P). We have identified five putative FYVE domain proteins in the genome of the protozoan parasite Leishmania major, three of which are predicted to contain a functional PtdIns(3)P-binding site. The FYVE domain of one of these proteins, LmFYVE-1, bound PtdIns(3)P in liposome-binding assays and targeted GFP to acidified late endosomes/lysosomes in mammalian cells. The high-resolution solution structure of its N-terminal FYVE domain (LmFYVE-1[1-79]) was solved by nuclear magnetic resonance. Functionally significant clusters of residues of the LmFYVE-1 domain involved in PtdIns(3)P binding and dependence on low pH for tight binding were identified. This structure is the first trypanosomatid membrane trafficking protein to be determined and has been refined to high precision and accuracy using residual dipolar couplings.
A high-resolution solution structure of a trypanosomatid FYVE domain.,Mertens HD, Callaghan JM, Swarbrick JD, McConville MJ, Gooley PR Protein Sci. 2007 Nov;16(11):2552-9. Epub 2007 Sep 28. PMID:17905827[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Mertens HD, Callaghan JM, Swarbrick JD, McConville MJ, Gooley PR. A high-resolution solution structure of a trypanosomatid FYVE domain. Protein Sci. 2007 Nov;16(11):2552-9. Epub 2007 Sep 28. PMID:17905827 doi:10.1110/ps.073009807
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