6xwy
From Proteopedia
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(New page: ==Highly pH-resistant long stokes-shift, red fluorescent protein mCRISPRed== <StructureSection load='6xwy' size='340' side='right'caption='6xwy, resolution 1.75Å'...) |
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<StructureSection load='6xwy' size='340' side='right'caption='[[6xwy]], [[Resolution|resolution]] 1.75Å' scene=''> | <StructureSection load='6xwy' size='340' side='right'caption='[[6xwy]], [[Resolution|resolution]] 1.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6xwy]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWY OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6xwy]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Entacmaea_quadricolor Entacmaea quadricolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6XWY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6XWY FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.75Å</td></tr> |
| - | <tr id=' | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MLA:MALONIC+ACID'>MLA</scene>, <scene name='pdbligand=NRQ:{(4Z)-4-(4-HYDROXYBENZYLIDENE)-2-[3-(METHYLTHIO)PROPANIMIDOYL]-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>NRQ</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6xwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6xwy OCA], [https://pdbe.org/6xwy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6xwy RCSB], [https://www.ebi.ac.uk/pdbsum/6xwy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6xwy ProSAT]</span></td></tr> |
</table> | </table> | ||
== Function == | == Function == | ||
| - | [ | + | [https://www.uniprot.org/uniprot/RFP_ENTQU RFP_ENTQU] Pigment protein.<ref>PMID:12185250</ref> |
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Engineered proteins must be phenotypically selected for function in the appropriate physiological context. Here, we present a versatile approach that allows generating panels of mammalian cells that express diversified heterologous protein libraries in the cytosol or subcellular compartments under stable conditions and in a single-variant-per-cell manner. To this end we adapt CRISPR/Cas9 editing technology to diversify targeted stretches of a protein of interest in situ. We demonstrate the utility of the approach by in situ engineering and intra-lysosome specific selection of an extremely pH-resistant long Stokes shift red fluorescent protein variant. Tailoring properties to specific conditions of cellular sub-compartments or organelles of mammalian cells can be an important asset to optimize various proteins, protein-based tools, and biosensors for distinct functions. | ||
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| + | Targeted In Situ Protein Diversification and Intra-organelle Validation in Mammalian Cells.,Erdogan M, Fabritius A, Basquin J, Griesbeck O Cell Chem Biol. 2020 Mar 3. pii: S2451-9456(20)30068-4. doi:, 10.1016/j.chembiol.2020.02.004. PMID:32142629<ref>PMID:32142629</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6xwy" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Entacmaea quadricolor]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Basquin | + | [[Category: Basquin J]] |
| - | [[Category: Erdogan | + | [[Category: Erdogan M]] |
| - | [[Category: Fabritius | + | [[Category: Fabritius A]] |
| - | [[Category: Griesbeck | + | [[Category: Griesbeck O]] |
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Current revision
Highly pH-resistant long stokes-shift, red fluorescent protein mCRISPRed
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