|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==NMR Structure of En-6 pheromone from the Antarctic Ciliate Euplotes nobilii== | | ==NMR Structure of En-6 pheromone from the Antarctic Ciliate Euplotes nobilii== |
| - | <StructureSection load='2jms' size='340' side='right'caption='[[2jms]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2jms' size='340' side='right'caption='[[2jms]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2jms]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Euplotes_nobilii Euplotes nobilii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2JMS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2jms]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Euplotes_nobilii Euplotes nobilii]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2JMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2JMS FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jms OCA], [http://pdbe.org/2jms PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2jms RCSB], [http://www.ebi.ac.uk/pdbsum/2jms PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2jms ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2jms FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jms OCA], [https://pdbe.org/2jms PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2jms RCSB], [https://www.ebi.ac.uk/pdbsum/2jms PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2jms ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0FKY4_EUPNO A0FKY4_EUPNO] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 21: |
Line 24: |
| | [[Category: Euplotes nobilii]] | | [[Category: Euplotes nobilii]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Alimenti, C]] | + | [[Category: Alimenti C]] |
| - | [[Category: Koculi, E]] | + | [[Category: Koculi E]] |
| - | [[Category: LaTerza, A]] | + | [[Category: LaTerza A]] |
| - | [[Category: Luporini, P]] | + | [[Category: Luporini P]] |
| - | [[Category: Pedrini, B]] | + | [[Category: Pedrini B]] |
| - | [[Category: Placzek, W J]] | + | [[Category: Placzek WJ]] |
| - | [[Category: Wuthrich, K]] | + | [[Category: Wuthrich K]] |
| - | [[Category: Protein]]
| + | |
| - | [[Category: Signaling protein]]
| + | |
| Structural highlights
Function
A0FKY4_EUPNO
Publication Abstract from PubMed
Ciliates of Euplotes species constitutively secrete pleiotropic protein pheromones, which are capable to function as prototypic autocrine growth factors as well as paracrine inducers of mating processes. This paper reports the amino acid sequence and the NMR structure of the pheromone En-6 isolated from the antarctic species Euplotes nobilii. The 63-residue En-6 polypeptide chain forms three alpha-helices in positions 18-25, 36-40 and 46-56, which are arranged in an up-down-up three-helix bundle forming the edges of a distorted trigonal pyramid. The base of the pyramid is covered by the N-terminal heptadecapeptide segment, which includes a 3(10)-turn of residues 3-6. This topology is covalently anchored by four long-range disulfide bonds. Comparison with the smaller pheromones of E. raikovi, a closely related species living in temperate waters, shows that the two-pheromone families have the same three-helix bundle architecture. It then appears that cold-adaptation of the En proteins is primarily related to increased lengths of the chain-terminal peptide segments and the surface-exposed loops connecting the regular secondary structures, and to the presence of solvent-exposed clusters of negatively charged side-chains.
Cold-adaptation in sea-water-borne signal proteins: sequence and NMR structure of the pheromone En-6 from the Antarctic ciliate Euplotes nobilii.,Pedrini B, Placzek WJ, Koculi E, Alimenti C, LaTerza A, Luporini P, Wuthrich K J Mol Biol. 2007 Sep 14;372(2):277-86. Epub 2007 Jun 26. PMID:17663000[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Pedrini B, Placzek WJ, Koculi E, Alimenti C, LaTerza A, Luporini P, Wuthrich K. Cold-adaptation in sea-water-borne signal proteins: sequence and NMR structure of the pheromone En-6 from the Antarctic ciliate Euplotes nobilii. J Mol Biol. 2007 Sep 14;372(2):277-86. Epub 2007 Jun 26. PMID:17663000 doi:S0022-2836(07)00841-8
|
