|
|
| (2 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| | | | |
| | ==Solution Structure of the Chemokine CCL21== | | ==Solution Structure of the Chemokine CCL21== |
| - | <StructureSection load='2l4n' size='340' side='right'caption='[[2l4n]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2l4n' size='340' side='right'caption='[[2l4n]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2l4n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2L4N FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2l4n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2L4N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2L4N FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CCL21, RP11-195F19.22-001, SCYA21, UNQ784/PRO1600 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2l4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l4n OCA], [http://pdbe.org/2l4n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2l4n RCSB], [http://www.ebi.ac.uk/pdbsum/2l4n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2l4n ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2l4n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2l4n OCA], [https://pdbe.org/2l4n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2l4n RCSB], [https://www.ebi.ac.uk/pdbsum/2l4n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2l4n ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/CCL21_HUMAN CCL21_HUMAN]] Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. | + | [https://www.uniprot.org/uniprot/CCL21_HUMAN CCL21_HUMAN] Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 22: |
Line 22: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Love, M]] | + | [[Category: Love M]] |
| - | [[Category: Peterson, F C]] | + | [[Category: Peterson FC]] |
| - | [[Category: Sandberg, J L]] | + | [[Category: Sandberg JL]] |
| - | [[Category: Veldkamp, C T]] | + | [[Category: Veldkamp CT]] |
| - | [[Category: Ccr7]]
| + | |
| - | [[Category: Chemokine]]
| + | |
| - | [[Category: Cytokine]]
| + | |
| - | [[Category: Exodus-2]]
| + | |
| - | [[Category: Slc]]
| + | |
| Structural highlights
Function
CCL21_HUMAN Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Publication Abstract from PubMed
CCL21 is a human chemokine that recruits normal immune cells and metastasizing tumor cells to lymph nodes through activation of the G protein-coupled receptor CCR7. The CCL21 structure solved by NMR contains a conserved chemokine domain followed by an extended, unstructured C-terminus that is not typical of most other chemokines. A sedimentation equilibrium study showed CCL21 to be monomeric. Chemical shift mapping indicates that the CCR7 N-terminus binds to the N-loop and third beta-strand of CCL21's chemokine domain. Details of CCL21-receptor recognition may enable structure-based drug discovery of novel antimetastatic agents.
Solution structure of CCL21 and identification of a putative CCR7 binding site.,Love M, Sandberg JL, Ziarek JJ, Gerarden KP, Rode RR, Jensen DR, McCaslin DR, Peterson FC, Veldkamp CT Biochemistry. 2012 Jan 24;51(3):733-5. Epub 2012 Jan 17. PMID:22221265[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Love M, Sandberg JL, Ziarek JJ, Gerarden KP, Rode RR, Jensen DR, McCaslin DR, Peterson FC, Veldkamp CT. Solution structure of CCL21 and identification of a putative CCR7 binding site. Biochemistry. 2012 Jan 24;51(3):733-5. Epub 2012 Jan 17. PMID:22221265 doi:http://dx.doi.org/10.1021/bi201601k
|
