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| <StructureSection load='6jn1' size='340' side='right'caption='[[6jn1]], [[Resolution|resolution]] 2.38Å' scene=''> | | <StructureSection load='6jn1' size='340' side='right'caption='[[6jn1]], [[Resolution|resolution]] 2.38Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
- | <table><tr><td colspan='2'>[[6jn1]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"campylobacter_fetus_subsp._jejuni"_smibert_1974 "campylobacter fetus subsp. jejuni" smibert 1974]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JN1 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6JN1 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6jn1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni Campylobacter jejuni] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6JN1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6JN1 FirstGlance]. <br> |
- | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.382Å</td></tr> |
- | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=C0O:'>C0O</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C0O:(~{Z},2~{R},6~{S})-2,6-bis(azanyl)hept-3-enedioic+acid'>C0O</scene>, <scene name='pdbligand=DAL:D-ALANINE'>DAL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
- | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">A8118_01115 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=197 "Campylobacter fetus subsp. jejuni" Smibert 1974])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6jn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jn1 OCA], [https://pdbe.org/6jn1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6jn1 RCSB], [https://www.ebi.ac.uk/pdbsum/6jn1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6jn1 ProSAT]</span></td></tr> |
- | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6jn1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6jn1 OCA], [http://pdbe.org/6jn1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6jn1 RCSB], [http://www.ebi.ac.uk/pdbsum/6jn1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6jn1 ProSAT]</span></td></tr> | + | |
| </table> | | </table> |
| + | == Function == |
| + | [https://www.uniprot.org/uniprot/A0A1J6PWI8_CAMJU A0A1J6PWI8_CAMJU] |
| <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
- | [[Category: Campylobacter fetus subsp. jejuni smibert 1974]] | + | [[Category: Campylobacter jejuni]] |
| [[Category: Large Structures]] | | [[Category: Large Structures]] |
- | [[Category: An, D R]] | + | [[Category: Synthetic construct]] |
- | [[Category: Lee, H H]] | + | [[Category: An DR]] |
- | [[Category: Min, K J]] | + | [[Category: Lee HH]] |
- | [[Category: Suh, S W]] | + | [[Category: Min KJ]] |
- | [[Category: Yoon, H J]] | + | [[Category: Suh SW]] |
- | [[Category: Hydrolase]] | + | [[Category: Yoon HJ]] |
- | [[Category: Peptidoglycan]]
| + | |
| Structural highlights
Function
A0A1J6PWI8_CAMJU
Publication Abstract from PubMed
Assembly of the peptidoglycan is crucial in maintaining viability of bacteria and in defining bacterial cell shapes, both of which are important for existence in the ecological niche that the organism occupies. Here, eight crystal structures for a member of the cell-shape-determining class of Campylobacter jejuni, the peptidoglycan peptidase 3 (Pgp3), are reported. Characterization of the turnover chemistry of Pgp3 reveals cell wall D,D-endopeptidase and D,D-carboxypeptidase activities. Catalysis is accompanied by large conformational changes upon peptidoglycan binding, whereby a loop regulates access to the active site. Furthermore, prior hydrolysis of the crosslinked peptide stem from the saccharide backbone of the peptidoglycan on one side is a pre-requisite for its recognition and turnover by Pgp3. These analyses reveal the noncanonical nature of the transformations at the core of the events that define the morphological shape for C. jejuni as an intestinal pathogen.
Peptidoglycan reshaping by a noncanonical peptidase for helical cell shape in Campylobacter jejuni.,Min K, An DR, Yoon HJ, Rana N, Park JS, Kim J, Lee M, Hesek D, Ryu S, Kim BM, Mobashery S, Suh SW, Lee HH Nat Commun. 2020 Jan 23;11(1):458. doi: 10.1038/s41467-019-13934-4. PMID:31974386[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Min K, An DR, Yoon HJ, Rana N, Park JS, Kim J, Lee M, Hesek D, Ryu S, Kim BM, Mobashery S, Suh SW, Lee HH. Peptidoglycan reshaping by a noncanonical peptidase for helical cell shape in Campylobacter jejuni. Nat Commun. 2020 Jan 23;11(1):458. doi: 10.1038/s41467-019-13934-4. PMID:31974386 doi:http://dx.doi.org/10.1038/s41467-019-13934-4
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