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| | <StructureSection load='6ugl' size='340' side='right'caption='[[6ugl]], [[Resolution|resolution]] 2.03Å' scene=''> | | <StructureSection load='6ugl' size='340' side='right'caption='[[6ugl]], [[Resolution|resolution]] 2.03Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ugl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Vibrio_cholerae_2010el-1786 Vibrio cholerae 2010el-1786]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UGL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UGL FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ugl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae_O1_str._2010EL-1786 Vibrio cholerae O1 str. 2010EL-1786]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UGL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UGL FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=QOS:3,5-dimethylpyrazin-2(1H)-one'>QOS</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.03Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">C9J66_03310, EC575_07960, EN12_18880, ERS013215_03763, EYB64_07770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=914149 Vibrio cholerae 2010EL-1786])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=QOS:3,5-dimethylpyrazin-2(1H)-one'>QOS</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ugl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ugl OCA], [http://pdbe.org/6ugl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ugl RCSB], [http://www.ebi.ac.uk/pdbsum/6ugl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ugl ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ugl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ugl OCA], [https://pdbe.org/6ugl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ugl RCSB], [https://www.ebi.ac.uk/pdbsum/6ugl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ugl ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A0F0AZW0_VIBCL A0A0F0AZW0_VIBCL] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6ugl" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6ugl" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Transcriptional activator 3D structures|Transcriptional activator 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Vibrio cholerae 2010el-1786]] | + | [[Category: Vibrio cholerae O1 str. 2010EL-1786]] |
| - | [[Category: Huang, X]] | + | [[Category: Huang X]] |
| - | [[Category: Paczkowski, J E]] | + | [[Category: Paczkowski JE]] |
| - | [[Category: Transcription]]
| + | |
| - | [[Category: Transcriptional activator protein of quorum sensing gene]]
| + | |
| Structural highlights
Function
A0A0F0AZW0_VIBCL
Publication Abstract from PubMed
Quorum sensing is a bacterial communication process whereby bacteria produce, release, and detect extracellular signaling molecules called autoinducers to coordinate collective behaviors. In the pathogen Vibrio cholerae, the quorum-sensing autoinducer 3,5-dimethyl-pyrazin-2-ol (DPO) binds the receptor and transcription factor VqmA. The DPO-VqmA complex activates transcription of vqmR, encoding the VqmR small RNA, which represses genes required for biofilm formation and virulence factor production. Here, we show that VqmA is soluble and properly folded, and activates basal-level transcription of its target vqmR in the absence of DPO. VqmA transcriptional activity is increased in response to increasing concentrations of DPO, allowing VqmA to drive the V. cholerae quorum-sensing transition at high cell densities. We solved the DPO-VqmA crystal structure to 2.0 A resolution and compared it to existing structures to understand the conformational changes VqmA undergoes upon DNA binding. Analysis of DPO analogs showed that a hydroxyl or carbonyl group at the 2' position is critical for binding to VqmA. The proposed DPO precursor, a linear molecule, N-alanyl-aminoacetone or Ala-AA, also bound and activated VqmA. Results from site-directed mutagenesis and competitive ligand-binding analyses revealed that DPO and Ala-AA occupy the same binding site. In summary, our structure-function analysis identifies key features required for VqmA activation and DNA binding and establishes that, while VqmA binds two different ligands, VqmA does not require a bound ligand for folding or basal transcriptional activity. However, bound ligand is required for maximal activity.
Mechanism underlying autoinducer recognition in the Vibrio cholerae DPO-VqmA quorum-sensing pathway.,Huang X, Duddy OP, Silpe JE, Paczkowski JE, Cong J, Henke BR, Bassler BL J Biol Chem. 2020 Jan 21. pii: RA119.012104. doi: 10.1074/jbc.RA119.012104. PMID:31964715[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Huang X, Duddy OP, Silpe JE, Paczkowski JE, Cong J, Henke BR, Bassler BL. Mechanism underlying autoinducer recognition in the Vibrio cholerae DPO-VqmA quorum-sensing pathway. J Biol Chem. 2020 Jan 21. pii: RA119.012104. doi: 10.1074/jbc.RA119.012104. PMID:31964715 doi:http://dx.doi.org/10.1074/jbc.RA119.012104
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