|
|
| (One intermediate revision not shown.) |
| Line 3: |
Line 3: |
| | <StructureSection load='1ztx' size='340' side='right'caption='[[1ztx]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='1ztx' size='340' side='right'caption='[[1ztx]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[1ztx]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [http://en.wikipedia.org/wiki/Wnv Wnv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZTX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ZTX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[1ztx]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus] and [https://en.wikipedia.org/wiki/West_Nile_virus West Nile virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZTX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1ZTX FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ztx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ztx OCA], [http://pdbe.org/1ztx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ztx RCSB], [http://www.ebi.ac.uk/pdbsum/1ztx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ztx ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1ztx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ztx OCA], [https://pdbe.org/1ztx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1ztx RCSB], [https://www.ebi.ac.uk/pdbsum/1ztx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1ztx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q91KZ4_WNV Q91KZ4_WNV] |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
| Line 34: |
Line 37: |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Mus musculus]] | | [[Category: Mus musculus]] |
| - | [[Category: Wnv]] | + | [[Category: West Nile virus]] |
| - | [[Category: Diamond, M S]] | + | [[Category: Diamond MS]] |
| - | [[Category: Fremont, D H]] | + | [[Category: Fremont DH]] |
| - | [[Category: Nybakken, G E]] | + | [[Category: Nybakken GE]] |
| - | [[Category: Oliphant, T]] | + | [[Category: Oliphant T]] |
| - | [[Category: Antibody]]
| + | |
| - | [[Category: Envelope]]
| + | |
| - | [[Category: Fab]]
| + | |
| - | [[Category: Neutralizing]]
| + | |
| - | [[Category: Viral protein-immune system complex]]
| + | |
| - | [[Category: Virus]]
| + | |
| Structural highlights
Function
Q91KZ4_WNV
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
West Nile virus is a mosquito-borne flavivirus closely related to the human epidemic-causing dengue, yellow fever and Japanese encephalitis viruses. In establishing infection these icosahedral viruses undergo endosomal membrane fusion catalysed by envelope glycoprotein rearrangement of the putative receptor-binding domain III (DIII) and exposure of the hydrophobic fusion loop. Humoral immunity has an essential protective function early in the course of West Nile virus infection. Here, we investigate the mechanism of neutralization by the E16 monoclonal antibody that specifically binds DIII. Structurally, the E16 antibody Fab fragment engages 16 residues positioned on four loops of DIII, a consensus neutralizing epitope sequence conserved in West Nile virus and distinct in other flaviviruses. The E16 epitope protrudes from the surface of mature virions in three distinct environments, and docking studies predict Fab binding will leave five-fold clustered epitopes exposed. We also show that E16 inhibits infection primarily at a step after viral attachment, potentially by blocking envelope glycoprotein conformational changes. Collectively, our results suggest that a vaccine strategy targeting the dominant DIII epitope may elicit safe and effective immune responses against flaviviral diseases.
Structural basis of West Nile virus neutralization by a therapeutic antibody.,Nybakken GE, Oliphant T, Johnson S, Burke S, Diamond MS, Fremont DH Nature. 2005 Sep 29;437(7059):764-9. PMID:16193056[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Nybakken GE, Oliphant T, Johnson S, Burke S, Diamond MS, Fremont DH. Structural basis of West Nile virus neutralization by a therapeutic antibody. Nature. 2005 Sep 29;437(7059):764-9. PMID:16193056 doi:10.1038/nature03956
|
