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| | <StructureSection load='4ckm' size='340' side='right'caption='[[4ckm]], [[Resolution|resolution]] 2.15Å' scene=''> | | <StructureSection load='4ckm' size='340' side='right'caption='[[4ckm]], [[Resolution|resolution]] 2.15Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4ckm]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Leima Leima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CKM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CKM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4ckm]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Leishmania_major Leishmania major]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CKM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4CKM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.15Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4ckn|4ckn]], [[4ckp|4ckp]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ckm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ckm OCA], [http://pdbe.org/4ckm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ckm RCSB], [http://www.ebi.ac.uk/pdbsum/4ckm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ckm ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ckm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ckm OCA], [https://pdbe.org/4ckm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ckm RCSB], [https://www.ebi.ac.uk/pdbsum/4ckm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ckm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/E9AFQ5_LEIMA E9AFQ5_LEIMA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Leima]] | + | [[Category: Leishmania major]] |
| - | [[Category: Breugel, M van]] | + | [[Category: Van Breugel M]] |
| - | [[Category: Basal body]]
| + | |
| - | [[Category: Cartwheel]]
| + | |
| - | [[Category: Centriole]]
| + | |
| - | [[Category: Structural protein]]
| + | |
| - | [[Category: Trypanosomatid]]
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| Structural highlights
Function
E9AFQ5_LEIMA
Publication Abstract from PubMed
Centrioles are cylindrical cell organelles with a ninefold symmetric peripheral microtubule array that is essential to template cilia and flagella. They are built around a central cartwheel assembly that is organized through homo-oligomerization of the centriolar protein SAS-6, but whether SAS-6 self-assembly can dictate cartwheel and thereby centriole symmetry is unclear. Here we show that Leishmania major SAS-6 crystallizes as a 9-fold symmetric cartwheel and provide the X-ray structure of this assembly at a resolution of 3.5 A. We furthermore demonstrate that oligomerization of Leishmania SAS-6 can be inhibited by a small molecule in vitro and provide indications for its binding site. Our results firmly establish that SAS-6 can impose cartwheel symmetry on its own and indicate how this process might occur mechanistically in vivo. Importantly, our data also provide a proof-of-principle that inhibition of SAS-6 oligomerization by small molecules is feasible. DOI: http://dx.doi.org/10.7554/eLife.01812.001.
Structure of the SAS-6 cartwheel hub from Leishmania major.,van Breugel M, Wilcken R, McLaughlin SH, Rutherford TJ, Johnson CM Elife. 2014 Jan 1;3:e01812. doi: 10.7554/eLife.01812. PMID:24596152[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ van Breugel M, Wilcken R, McLaughlin SH, Rutherford TJ, Johnson CM. Structure of the SAS-6 cartwheel hub from Leishmania major. Elife. 2014 Jan 1;3:e01812. doi: 10.7554/eLife.01812. PMID:24596152
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