6rwy
From Proteopedia
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==Export apparatus core and inner rod of the Shigella type 3 secretion system== | ==Export apparatus core and inner rod of the Shigella type 3 secretion system== | ||
| - | < | + | <SX load='6rwy' size='340' side='right' viewer='molstar' caption='[[6rwy]], [[Resolution|resolution]] 5.11Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6rwy]] is a 33 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RWY OCA]. For a <b>guided tour on the structure components</b> use [http:// | + | <table><tr><td colspan='2'>[[6rwy]] is a 33 chain structure with sequence from [http://en.wikipedia.org/wiki/"shigella_paradysenteriae"_weldin_1927 "shigella paradysenteriae" weldin 1927] and [http://en.wikipedia.org/wiki/Shigella_flexneri Shigella flexneri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6RWY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6RWY FirstGlance]. <br> |
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=UNK:UNKNOWN'>UNK</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6rwx|6rwx]], [[6rwk|6rwk]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6rwx|6rwx]], [[6rwk|6rwk]]</td></tr> | ||
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http:// | + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mxiH, CP0137 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=623 "Shigella paradysenteriae" Weldin 1927])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6rwy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6rwy OCA], [http://pdbe.org/6rwy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6rwy RCSB], [http://www.ebi.ac.uk/pdbsum/6rwy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6rwy ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/SPAQ_SHIFL SPAQ_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the Ipa antigens. Required for invasion and for secretion of the three Ipa proteins. [[http://www.uniprot.org/uniprot/SPAP_SHIFL SPAP_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three Ipa proteins. [[http://www.uniprot.org/uniprot/MXIH_SHIFL MXIH_SHIFL]] Necessary for the secretion of IPA invasins. [[http://www.uniprot.org/uniprot/SPAR_SHIFL SPAR_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three ipa proteins. | [[http://www.uniprot.org/uniprot/SPAQ_SHIFL SPAQ_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the Ipa antigens. Required for invasion and for secretion of the three Ipa proteins. [[http://www.uniprot.org/uniprot/SPAP_SHIFL SPAP_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three Ipa proteins. [[http://www.uniprot.org/uniprot/MXIH_SHIFL MXIH_SHIFL]] Necessary for the secretion of IPA invasins. [[http://www.uniprot.org/uniprot/SPAR_SHIFL SPAR_SHIFL]] Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three ipa proteins. | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Type III Secretion Systems (T3SS) needle complex is a conserved syringe-shaped protein translocation nanomachine with a mass of about 3.5 MDa essential for the survival and virulence of many Gram-negative bacterial pathogens. This system is composed of a membrane-embedded basal body and an extracellular needle that deliver effector proteins into host cells. High-resolution structures of the T3SS from different organisms and infection stages are needed to understand the underlying molecular mechanisms of effector translocation. Here, we present the cryo-electron microscopy structure of the isolated Shigella T3SS needle complex. The inner membrane (IM) region of the basal body adopts 24-fold rotational symmetry and forms a channel system that connects the bacterial periplasm with the export apparatus cage. The secretin oligomer adopts a heterogeneous architecture with 16- and 15-fold cyclic symmetry in the periplasmic N-terminal connector and C-terminal outer membrane ring, respectively. Two out of three IM subunits bind the secretin connector via a beta-sheet augmentation. The cryo-EM map also reveals the helical architecture of the export apparatus core, the inner rod, the needle and their intervening interfaces. | ||
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| + | Cryo-EM structure of the Shigella type III needle complex.,Lunelli M, Kamprad A, Burger J, Mielke T, Spahn CMT, Kolbe M PLoS Pathog. 2020 Feb 24;16(2):e1008263. doi: 10.1371/journal.ppat.1008263. PMID:32092125<ref>PMID:32092125</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6rwy" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
| - | </ | + | </SX> |
| + | [[Category: Shigella paradysenteriae weldin 1927]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Shigella flexneri]] | [[Category: Shigella flexneri]] | ||
Current revision
Export apparatus core and inner rod of the Shigella type 3 secretion system
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