4ki2

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<StructureSection load='4ki2' size='340' side='right'caption='[[4ki2]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
<StructureSection load='4ki2' size='340' side='right'caption='[[4ki2]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[4ki2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_25104 Atcc 25104]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4gor 4gor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KI2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KI2 FirstGlance]. <br>
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<table><tr><td colspan='2'>[[4ki2]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_aquaticus Thermus aquaticus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4gor 4gor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KI2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KI2 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.3&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3ugo|3ugo]], [[3ugp|3ugp]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4ki2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ki2 OCA], [http://pdbe.org/4ki2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4ki2 RCSB], [http://www.ebi.ac.uk/pdbsum/4ki2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4ki2 ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ki2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ki2 OCA], [https://pdbe.org/4ki2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ki2 RCSB], [https://www.ebi.ac.uk/pdbsum/4ki2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ki2 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
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[[http://www.uniprot.org/uniprot/Q9EZJ8_THEAQ Q9EZJ8_THEAQ]] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released (By similarity).[RuleBase:RU000715]
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[https://www.uniprot.org/uniprot/SIGA_THEAQ SIGA_THEAQ] Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is the primary sigma factor during exponential growth.[HAMAP-Rule:MF_00963]<ref>PMID:11114902</ref> <ref>PMID:11931761</ref>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Structural studies of -10 promoter element recognition by domain 2 of the RNA polymerase sigma subunit [Feklistov &amp; Darst (2011), Cell, 147, 1257-1269] reveal an unusual crystal-packing arrangement dominated by G-quartets. The 3'-terminal GGG motif of the oligonucleotide used in crystallization participates in G-quadruplex formation with GGG motifs from symmetry-related complexes. Stacking between neighboring G-quadruplexes results in the formation of pseudo-continuous four-stranded columns running throughout the length of the crystal (G-columns). Here, a new crystal form is presented with a different arrangement of G-columns and it is proposed that the fortuitous finding of G-quartet packing could be useful in engineering crystal contacts in protein-ssDNA complexes.
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Crystallographic analysis of an RNA polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA: quadruplex formation as a possible tool for engineering crystal contacts in protein-ssDNA complexes.,Feklistov A, Darst SA Acta Crystallogr Sect F Struct Biol Cryst Commun. 2013 Sep;69(Pt 9):950-5. doi:, 10.1107/S1744309113020368. Epub 2013 Aug 19. PMID:23989139<ref>PMID:23989139</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ki2" style="background-color:#fffaf0;"></div>
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==See Also==
==See Also==
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Atcc 25104]]
 
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Darst, S A]]
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[[Category: Thermus aquaticus]]
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[[Category: Feklistov, A]]
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[[Category: Darst SA]]
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[[Category: G-quadruplex]]
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[[Category: Feklistov A]]
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[[Category: G-quartet]]
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[[Category: Promoter opening]]
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[[Category: Promoter recognition]]
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[[Category: Protein-dna binding]]
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[[Category: Ssdna]]
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[[Category: Ssdna-binding]]
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[[Category: Transcription initiation]]
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[[Category: Transcription-dna complex]]
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Current revision

Crystallographic analysis of an RNA-polymerase sigma-subunit fragment complexed with -10 promoter element ssDNA

PDB ID 4ki2

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