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| | ==Monomeric parallel-stranded DNA tetraplex with snap-back 3+1 3' G-tetrad, single-residue chain reversal loops, GAG triad in the context of GAAG diagonal loop, NMR, 8 struct.== | | ==Monomeric parallel-stranded DNA tetraplex with snap-back 3+1 3' G-tetrad, single-residue chain reversal loops, GAG triad in the context of GAAG diagonal loop, NMR, 8 struct.== |
| - | <StructureSection load='2a5p' size='340' side='right'caption='[[2a5p]], [[NMR_Ensembles_of_Models | 8 NMR models]]' scene=''> | + | <StructureSection load='2a5p' size='340' side='right'caption='[[2a5p]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2a5p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5P OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A5P FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2a5p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A5P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2A5P FirstGlance]. <br> |
| - | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=DI:2-DEOXYINOSINE-5-MONOPHOSPHATE'>DI</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1eeg|1eeg]], [[1jjp|1jjp]], [[1y8d|1y8d]], [[1d6d|1d6d]], [[2a5r|2a5r]]</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2a5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a5p OCA], [https://pdbe.org/2a5p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2a5p RCSB], [https://www.ebi.ac.uk/pdbsum/2a5p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2a5p ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2a5p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2a5p OCA], [http://pdbe.org/2a5p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2a5p RCSB], [http://www.ebi.ac.uk/pdbsum/2a5p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2a5p ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | [[Category: Homo sapiens]] | | [[Category: Homo sapiens]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Gaw, H Y]] | + | [[Category: Gaw HY]] |
| - | [[Category: Kuryavyi, V V]] | + | [[Category: Kuryavyi VV]] |
| - | [[Category: Patel, D J]] | + | [[Category: Patel DJ]] |
| - | [[Category: Phan, A T]] | + | [[Category: Phan AT]] |
| - | [[Category: C-myc promoter 3+1 g-tetrad]]
| + | |
| - | [[Category: Dna]]
| + | |
| - | [[Category: Gaag loop]]
| + | |
| - | [[Category: Gag triad]]
| + | |
| - | [[Category: Monomeric parallel-stranded quadruplex]]
| + | |
| - | [[Category: Single nucleotide chain reversal loop]]
| + | |
| Structural highlights
Publication Abstract from PubMed
It has been widely accepted that DNA can adopt other biologically relevant structures beside the Watson-Crick double helix. One recent important example is the guanine-quadruplex (G-quadruplex) structure formed by guanine tracts found in the MYC (or c-myc) promoter region, which regulates the transcription of the MYC oncogene. Stabilization of this G-quadruplex by ligands, such as the cationic porphyrin TMPyP4, decreases the transcriptional level of MYC. Here, we report the first structure of a DNA fragment containing five guanine tracts from this region. An unusual G-quadruplex fold, which was derived from NMR restraints using unambiguous model-independent resonance assignment approaches, involves a core of three stacked guanine tetrads formed by four parallel guanine tracts with all anti guanines and a snapback 3'-end syn guanine. We have determined the structure of the complex formed between this G-quadruplex and TMPyP4. This structural information, combined with details of small-molecule interaction, provides a platform for the design of anticancer drugs targeting multi-guanine-tract sequences that are found in the MYC and other oncogenic promoters, as well as in telomeres.
Small-molecule interaction with a five-guanine-tract G-quadruplex structure from the human MYC promoter.,Phan AT, Kuryavyi V, Gaw HY, Patel DJ Nat Chem Biol. 2005 Aug;1(3):167-73. Epub 2005 Jul 17. PMID:16408022[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Phan AT, Kuryavyi V, Gaw HY, Patel DJ. Small-molecule interaction with a five-guanine-tract G-quadruplex structure from the human MYC promoter. Nat Chem Biol. 2005 Aug;1(3):167-73. Epub 2005 Jul 17. PMID:16408022 doi:nchembio723
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