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| | <StructureSection load='4o88' size='340' side='right'caption='[[4o88]], [[Resolution|resolution]] 2.90Å' scene=''> | | <StructureSection load='4o88' size='340' side='right'caption='[[4o88]], [[Resolution|resolution]] 2.90Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[4o88]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_22303 Atcc 22303]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O88 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4O88 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[4o88]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Millerozyma_acaciae Millerozyma acaciae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4O88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4O88 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.9Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4o87|4o87]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4o88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o88 OCA], [http://pdbe.org/4o88 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4o88 RCSB], [http://www.ebi.ac.uk/pdbsum/4o88 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4o88 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4o88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4o88 OCA], [https://pdbe.org/4o88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4o88 RCSB], [https://www.ebi.ac.uk/pdbsum/4o88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4o88 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| - | <div style="background-color:#fffaf0;">
| + | == Function == |
| - | == Publication Abstract from PubMed == | + | [https://www.uniprot.org/uniprot/Q707V3_9ASCO Q707V3_9ASCO] |
| - | tRNA restriction by anticodon nucleases underlies cellular stress responses and self-nonself discrimination in a wide range of taxa. Anticodon breakage inhibits protein synthesis, which, in turn, results in growth arrest or cell death. The eukaryal ribotoxin PaT secreted by Pichia acaciae inhibits growth of Saccharomyces cerevisiae via cleavage of tRNA(Gln(UUG)). We find that recombinant PaT incises a synthetic tRNA(Gln(UUG)) stem-loop RNA by transesterification at a single site 3' of the wobble uridine, yielding 2',3'-cyclic phosphate and 5'-OH ends. Incision is suppressed by replacement of the wobble nucleobase with adenine or guanine. The crystal structure of PaT reveals a distinctive fold and active site, essential components of which are demonstrated by mutagenesis. Pichia acaciae evades self-toxicity via a distinctive intracellular immunity protein, ImmPaT, which binds PaT and blocks nuclease activity. Our results highlight the evolutionary diversity of tRNA restriction and immunity systems.
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| - | Structure, mechanism, and specificity of a eukaryal tRNA restriction enzyme involved in self-nonself discrimination.,Chakravarty AK, Smith P, Jalan R, Shuman S Cell Rep. 2014 Apr 24;7(2):339-47. doi: 10.1016/j.celrep.2014.03.034. Epub 2014, Apr 13. PMID:24726365<ref>PMID:24726365</ref>
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| - | | + | |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | |
| - | </div>
| + | |
| - | <div class="pdbe-citations 4o88" style="background-color:#fffaf0;"></div>
| + | |
| - | == References ==
| + | |
| - | <references/>
| + | |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Atcc 22303]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Chakravarty, A K]] | + | [[Category: Millerozyma acaciae]] |
| - | [[Category: Shuman, S]] | + | [[Category: Chakravarty AK]] |
| - | [[Category: Smith, P]] | + | [[Category: Shuman S]] |
| - | [[Category: Hydrolase]] | + | [[Category: Smith P]] |
| - | [[Category: Novel fold]]
| + | |
| - | [[Category: Nuclease]]
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