|
|
| (12 intermediate revisions not shown.) |
| Line 1: |
Line 1: |
| - | [[Image:1aj8.gif|left|200px]] | |
| | | | |
| - | <!-- | + | ==CITRATE SYNTHASE FROM PYROCOCCUS FURIOSUS== |
| - | The line below this paragraph, containing "STRUCTURE_1aj8", creates the "Structure Box" on the page.
| + | <StructureSection load='1aj8' size='340' side='right'caption='[[1aj8]], [[Resolution|resolution]] 1.90Å' scene=''> |
| - | You may change the PDB parameter (which sets the PDB file loaded into the applet)
| + | == Structural highlights == |
| - | or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
| + | <table><tr><td colspan='2'>[[1aj8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJ8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1AJ8 FirstGlance]. <br> |
| - | or leave the SCENE parameter empty for the default display.
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| - | --> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr> |
| - | {{STRUCTURE_1aj8| PDB=1aj8 | SCENE= }}
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1aj8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1aj8 OCA], [https://pdbe.org/1aj8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1aj8 RCSB], [https://www.ebi.ac.uk/pdbsum/1aj8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1aj8 ProSAT]</span></td></tr> |
| | + | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/CISY_PYRFU CISY_PYRFU] |
| | + | == Evolutionary Conservation == |
| | + | [[Image:Consurf_key_small.gif|200px|right]] |
| | + | Check<jmol> |
| | + | <jmolCheckbox> |
| | + | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/aj/1aj8_consurf.spt"</scriptWhenChecked> |
| | + | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| | + | <text>to colour the structure by Evolutionary Conservation</text> |
| | + | </jmolCheckbox> |
| | + | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1aj8 ConSurf]. |
| | + | <div style="clear:both"></div> |
| | | | |
| - | '''CITRATE SYNTHASE FROM PYROCOCCUS FURIOSUS'''
| + | ==See Also== |
| - | | + | *[[Citrate Synthase 3D structures|Citrate Synthase 3D structures]] |
| - | | + | __TOC__ |
| - | ==Overview== | + | </StructureSection> |
| - | The crystal structure of the closed form of citrate synthase, with citrate and CoA bound, from the hyperthermophilic Archaeon Pyrococcus furiosus has been determined to 1.9 A. This has allowed direct structural comparisons between the same enzyme from organisms growing optimally at 37 degrees C (pig), 55 degrees C (Thermoplasma acidophilum) and now 100 degrees C (Pyrococcus furiosus). The three enzymes are homodimers and share a similar overall fold, with the dimer interface comprising primarily an eight alpha-helical sandwich of four antiparallel pairs of helices. The active sites show similar modes of substrate binding; moreover, the structural equivalence of the amino acid residues implicated in catalysis implies that the mechanism proceeds via the same acid-base catalytic process. Given the overall structural and mechanistic similarities, it has been possible to make detailed structural comparisons between the three citrate synthases, and a number of differences can be identified in passing from the mesophilic to thermophilic to hyperthermophilic citrate synthases. The most significant of these are an increased compactness of the enzyme, a more intimate association of the subunits, an increase in intersubunit ion pairs, and a reduction in thermolabile residues. Compactness is achieved by the shortening of a number of loops, an increase in the number of atoms buried from solvent, an optimized packing of side chains in the interior, and an absence of cavities. The intimate subunit association in the dimeric P. furiosus enzyme is achieved by greater complementarity of the monomers and by the C-terminal region of each monomer folding over the surface of the other monomer, in contrast to the pig enzyme where the C-terminus has a very different fold. The increased number of intersubunit ion pairs is accompanied by an increase in the number involved in networks. Interestingly, all loop regions in the P. furiosus enzyme either are shorter or contain additional ion pairs compared with the pig enzyme. The possible relevance of these structural features to enzyme hyperthermostability is discussed.
| + | [[Category: Large Structures]] |
| - | | + | |
| - | ==About this Structure==
| + | |
| - | 1AJ8 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Pyrococcus_furiosus Pyrococcus furiosus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1AJ8 OCA].
| + | |
| - | | + | |
| - | ==Reference==
| + | |
| - | The crystal structure of citrate synthase from the hyperthermophilic archaeon pyrococcus furiosus at 1.9 A resolution,., Russell RJ, Ferguson JM, Hough DW, Danson MJ, Taylor GL, Biochemistry. 1997 Aug 19;36(33):9983-94. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9254593 9254593]
| + | |
| | [[Category: Pyrococcus furiosus]] | | [[Category: Pyrococcus furiosus]] |
| - | [[Category: Single protein]]
| + | [[Category: Danson MJ]] |
| - | [[Category: Danson, M J.]] | + | [[Category: Ferguson JMC]] |
| - | [[Category: Ferguson, J M.C.]] | + | [[Category: Hough DW]] |
| - | [[Category: Hough, D W.]] | + | [[Category: Russell RJM]] |
| - | [[Category: Russell, R J.M.]] | + | [[Category: Taylor GL]] |
| - | [[Category: Taylor, G L.]] | + | |
| - | [[Category: Hyperthermostable]]
| + | |
| - | [[Category: Lyase]]
| + | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Fri May 2 10:20:34 2008''
| + | |
