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| | ==Solution NMR structure of toxin-like potassium channel blocking domain in MMP23== | | ==Solution NMR structure of toxin-like potassium channel blocking domain in MMP23== |
| - | <StructureSection load='2k72' size='340' side='right'caption='[[2k72]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2k72' size='340' side='right'caption='[[2k72]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2k72]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K72 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2K72 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2k72]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Rattus_norvegicus Rattus norvegicus]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2K72 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2K72 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2k72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k72 OCA], [http://pdbe.org/2k72 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2k72 RCSB], [http://www.ebi.ac.uk/pdbsum/2k72 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2k72 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2k72 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2k72 OCA], [https://pdbe.org/2k72 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2k72 RCSB], [https://www.ebi.ac.uk/pdbsum/2k72 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2k72 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MMP23_RAT MMP23_RAT]] Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum.<ref>PMID:19965868</ref> | + | [https://www.uniprot.org/uniprot/MMP23_RAT MMP23_RAT] Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum.<ref>PMID:19965868</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Feng, Z]] | + | [[Category: Rattus norvegicus]] |
| - | [[Category: Khoo, K K]] | + | [[Category: Feng Z]] |
| - | [[Category: Norton, R S]] | + | [[Category: Khoo KK]] |
| - | [[Category: Cleavage on pair of basic residue]] | + | [[Category: Norton RS]] |
| - | [[Category: Glycoprotein]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Immunoglobulin domain]]
| + | |
| - | [[Category: Membrane]]
| + | |
| - | [[Category: Metal-binding]]
| + | |
| - | [[Category: Metalloprotease]]
| + | |
| - | [[Category: Mmp23]]
| + | |
| - | [[Category: Potassium channel]]
| + | |
| - | [[Category: Signal-anchor]]
| + | |
| - | [[Category: Toxin]]
| + | |
| - | [[Category: Transmembrane]]
| + | |
| - | [[Category: Zinc]]
| + | |
| - | [[Category: Zymogen]]
| + | |
| Structural highlights
Function
MMP23_RAT Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum.[1]
Publication Abstract from PubMed
Peptide toxins found in a wide array of venoms block K(+) channels, causing profound physiological and pathological effects. Here we describe the first functional K(+) channel-blocking toxin domain in a mammalian protein. MMP23 (matrix metalloprotease 23) contains a domain (MMP23(TxD)) that is evolutionarily related to peptide toxins from sea anemones. MMP23(TxD) shows close structural similarity to the sea anemone toxins BgK and ShK. Moreover, this domain blocks K(+) channels in the nanomolar to low micromolar range (Kv1.6 > Kv1.3 > Kv1.1 = Kv3.2 > Kv1.4, in decreasing order of potency) while sparing other K(+) channels (Kv1.2, Kv1.5, Kv1.7, and KCa3.1). Full-length MMP23 suppresses K(+) channels by co-localizing with and trapping MMP23(TxD)-sensitive channels in the ER. Our results provide clues to the structure and function of the vast family of proteins that contain domains related to sea anemone toxins. Evolutionary pressure to maintain a channel-modulatory function may contribute to the conservation of this domain throughout the plant and animal kingdoms.
Potassium channel modulation by a toxin domain in matrix metalloprotease 23.,Rangaraju S, Khoo KK, Feng ZP, Crossley G, Nugent D, Khaytin I, Chi V, Pham C, Calabresi P, Pennington MW, Norton RS, Chandy KG J Biol Chem. 2010 Mar 19;285(12):9124-36. Epub 2009 Dec 4. PMID:19965868[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Rangaraju S, Khoo KK, Feng ZP, Crossley G, Nugent D, Khaytin I, Chi V, Pham C, Calabresi P, Pennington MW, Norton RS, Chandy KG. Potassium channel modulation by a toxin domain in matrix metalloprotease 23. J Biol Chem. 2010 Mar 19;285(12):9124-36. Epub 2009 Dec 4. PMID:19965868 doi:10.1074/jbc.M109.071266
- ↑ Rangaraju S, Khoo KK, Feng ZP, Crossley G, Nugent D, Khaytin I, Chi V, Pham C, Calabresi P, Pennington MW, Norton RS, Chandy KG. Potassium channel modulation by a toxin domain in matrix metalloprotease 23. J Biol Chem. 2010 Mar 19;285(12):9124-36. Epub 2009 Dec 4. PMID:19965868 doi:10.1074/jbc.M109.071266
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