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| | ==Solution structure of micelle-bound kalata B2== | | ==Solution structure of micelle-bound kalata B2== |
| - | <StructureSection load='2kch' size='340' side='right'caption='[[2kch]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='2kch' size='340' side='right'caption='[[2kch]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2kch]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Oldenlandia_affinis Oldenlandia affinis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KCH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2KCH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2kch]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Oldenlandia_affinis Oldenlandia affinis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2KCH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2KCH FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2kcg|2kcg]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR, 20 models</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2kch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kch OCA], [http://pdbe.org/2kch PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2kch RCSB], [http://www.ebi.ac.uk/pdbsum/2kch PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2kch ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2kch FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2kch OCA], [https://pdbe.org/2kch PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2kch RCSB], [https://www.ebi.ac.uk/pdbsum/2kch PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2kch ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/KAB2_OLDAF KAB2_OLDAF]] Probably participates in a plant defense mechanism. Inhibitory effect on the growth and development of larvae from Helicoverpa punctigera. Has hemolytic activity.<ref>PMID:15654741</ref> | + | [https://www.uniprot.org/uniprot/KAB2_OLDAF KAB2_OLDAF] Probably participates in a plant defense mechanism. Inhibitory effect on the growth and development of larvae from Helicoverpa punctigera. Has hemolytic activity.<ref>PMID:15654741</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Oldenlandia affinis]] | | [[Category: Oldenlandia affinis]] |
| - | [[Category: Wang, C K]] | + | [[Category: Wang CK]] |
| - | [[Category: Cyclic cystine knot]]
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| - | [[Category: Cytolysis]]
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| - | [[Category: Hemolysis]]
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| - | [[Category: Knottin]]
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| - | [[Category: Oxidation]]
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| - | [[Category: Plant defense]]
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| - | [[Category: Plant protein]]
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| Structural highlights
Function
KAB2_OLDAF Probably participates in a plant defense mechanism. Inhibitory effect on the growth and development of larvae from Helicoverpa punctigera. Has hemolytic activity.[1]
Publication Abstract from PubMed
Cyclotides are cyclic proteins produced by plants for defense against pests. Because of their remarkable stability and diverse bioactivities, they have a range of potential therapeutic applications. The bioactivities of cyclotides are believed to be mediated through membrane interactions. To determine the structural basis for the biological activity of the two major subfamilies of cyclotides, we determined the conformation and orientation of kalata B2 (kB2), a Mobius cyclotide, and cycloviolacin O2 (cO2), a bracelet cyclotide, bound to dodecylphosphocholine micelles, using NMR spectroscopy in the presence and absence of 5- and 16-doxylstearate relaxation probes. Analysis of binding curves using the Langmuir isotherm indicated that cO2 and kB2 have association constants of 7.0 x 10(3) M(-1) and 6.0 x 10(3) M(-1), respectively, consistent with the notion that they are bound near the surface, rather than buried deeply within the micelle. This suggestion is supported by the selective broadening of micelle-bound cyclotide NMR signals upon addition of paramagnetic Mn ions. The cyclotides from the different subfamilies exhibited clearly different binding orientations at the micelle surface. Structural analysis of cO2 confirmed that the main element of the secondary structure is a beta-hairpin centered in loop 5. A small helical turn is present in loop 3. Analysis of the surface profile of cO2 shows that a hydrophobic patch stretches over loops 2 and 3, in contrast to the hydrophobic patch of kB2, which predominantly involves loops 2 and 5. The different location of the hydrophobic patches in the two cyclotides explains their different binding orientations and provides an insight into the biological activities of cyclotides.
Despite a conserved cystine knot motif, different cyclotides have different membrane binding modes.,Wang CK, Colgrave ML, Ireland DC, Kaas Q, Craik DJ Biophys J. 2009 Sep 2;97(5):1471-81. PMID:19720036[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Jennings CV, Rosengren KJ, Daly NL, Plan M, Stevens J, Scanlon MJ, Waine C, Norman DG, Anderson MA, Craik DJ. Isolation, solution structure, and insecticidal activity of kalata B2, a circular protein with a twist: do Mobius strips exist in nature? Biochemistry. 2005 Jan 25;44(3):851-60. PMID:15654741 doi:10.1021/bi047837h
- ↑ Wang CK, Colgrave ML, Ireland DC, Kaas Q, Craik DJ. Despite a conserved cystine knot motif, different cyclotides have different membrane binding modes. Biophys J. 2009 Sep 2;97(5):1471-81. PMID:19720036 doi:10.1016/j.bpj.2009.06.032
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