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| | <StructureSection load='5cxm' size='340' side='right'caption='[[5cxm]], [[Resolution|resolution]] 1.70Å' scene=''> | | <StructureSection load='5cxm' size='340' side='right'caption='[[5cxm]], [[Resolution|resolution]] 1.70Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5cxm]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Syny3 Syny3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CXM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CXM FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5cxm]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Synechocystis_sp._PCC_6803_substr._Kazusa Synechocystis sp. PCC 6803 substr. Kazusa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CXM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5CXM FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">petC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1111708 SYNY3])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FES:FE2/S2+(INORGANIC)+CLUSTER'>FES</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5cxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cxm OCA], [http://pdbe.org/5cxm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5cxm RCSB], [http://www.ebi.ac.uk/pdbsum/5cxm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5cxm ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5cxm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5cxm OCA], [https://pdbe.org/5cxm PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5cxm RCSB], [https://www.ebi.ac.uk/pdbsum/5cxm PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5cxm ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/P74174_SYNY3 P74174_SYNY3] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Syny3]] | + | [[Category: Synechocystis sp. PCC 6803 substr. Kazusa]] |
| - | [[Category: Miki, K]] | + | [[Category: Miki K]] |
| - | [[Category: Roegner, M]] | + | [[Category: Roegner M]] |
| - | [[Category: Takeda, K]] | + | [[Category: Takeda K]] |
| - | [[Category: Veit, S]] | + | [[Category: Veit S]] |
| - | [[Category: 2fe-2s cluster]]
| + | |
| - | [[Category: Cyanobacteria]]
| + | |
| - | [[Category: Electron transport]]
| + | |
| - | [[Category: Lipoprotein]]
| + | |
| - | [[Category: Metal binding protein]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Petc3]]
| + | |
| - | [[Category: Plasma membrane]]
| + | |
| - | [[Category: Rieske protein]]
| + | |
| - | [[Category: Sll1182]]
| + | |
| - | [[Category: Synechocystis pcc 6803]]
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| Structural highlights
Function
P74174_SYNY3
Publication Abstract from PubMed
The cyanobacterium Synechocystis PCC 6803 possesses three Rieske isoforms: PetC1, PetC2 and PetC3. While PetC1 and PetC2 have been identified as alternative subunits of the cytochrome b6f complex (b6f), PetC3 was localized exclusively within the plasma membrane. The spatial separation of PetC3 from the photosynthetic and respiratory protein complexes raises doubt in its involvement in bioenergetic electron transfer. Here we report a detailed structural and functional characterization of the cyanobacterial PetC3 protein family indicating that PetC3 is not a component of the b6f and the photosynthetic electron transport as implied by gene annotation. Instead PetC3 has a distinct function in cell envelope homeostasis. Especially proteomic analysis shows that deletion of petC3 in Synechocystis PCC 6803 primarily affects cell envelope proteins including many nutrient transport systems. Therefore, the observed downregulation in the photosynthetic electron transport - mainly caused by photosystem 2 inactivation - might constitute a stress adaptation. Comprehensive in silico sequence analyses revealed that PetC3 proteins are periplasmic lipoproteins tethered to the plasma membrane with a subclass consisting of soluble periplasmic proteins, i.e. their N-terminal domain is inconsistent with their integration into the b6f. For the first time, the structure of PetC3 was determined by X-ray crystallography at an atomic resolution revealing significant high similarities to non-b6f Rieske subunits in contrast to PetC1. These results suggest that PetC3 affects processes in the periplasmic compartment that only indirectly influence photosynthetic electron transport. For this reason, we suggest to rename "Photosynthetic electron transport Chain 3" (PetC3) proteins as "periplasmic Rieske proteins" (Prp).
Structural and functional characterisation of the cyanobacterial PetC3 Rieske protein family.,Veit S, Takeda K, Tsunoyama Y, Baymann F, Nevo R, Reich Z, Rogner M, Miki K, Rexroth S Biochim Biophys Acta. 2016 Sep 20. pii: S0005-2728(16)30622-3. doi:, 10.1016/j.bbabio.2016.09.007. PMID:27663073[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Veit S, Takeda K, Tsunoyama Y, Baymann F, Nevo R, Reich Z, Rogner M, Miki K, Rexroth S. Structural and functional characterisation of the cyanobacterial PetC3 Rieske protein family. Biochim Biophys Acta. 2016 Sep 20. pii: S0005-2728(16)30622-3. doi:, 10.1016/j.bbabio.2016.09.007. PMID:27663073 doi:http://dx.doi.org/10.1016/j.bbabio.2016.09.007
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