5idb

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<StructureSection load='5idb' size='340' side='right'caption='[[5idb]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
<StructureSection load='5idb' size='340' side='right'caption='[[5idb]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5idb]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Cragi Cragi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IDB OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5IDB FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5idb]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Crassostrea_gigas Crassostrea gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5IDB OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5IDB FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5id8|5id8]], [[5ida|5ida]]</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NHE:2-[N-CYCLOHEXYLAMINO]ETHANE+SULFONIC+ACID'>NHE</scene></td></tr>
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<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CGI_10011001, CGI_10018577 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29159 CRAGI])</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5idb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5idb OCA], [https://pdbe.org/5idb PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5idb RCSB], [https://www.ebi.ac.uk/pdbsum/5idb PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5idb ProSAT]</span></td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5idb FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5idb OCA], [http://pdbe.org/5idb PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5idb RCSB], [http://www.ebi.ac.uk/pdbsum/5idb PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5idb ProSAT]</span></td></tr>
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</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/K1QRB6_CRAGI K1QRB6_CRAGI]
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A novel mannose-specific lectin, named CGL1 (15.5 kDa), was isolated from the oyster Crassostrea gigas. Characterization of CGL1 involved isothermal titration calorimetry (ITC), glycoconjugate microarray, and frontal affinity chromatography (FAC). This analysis revealed that CGL1 has strict specificity for the mannose monomer and for high mannose-type N-glycans (HMTGs). Primary structure of CGL1 did not show any homology with known lectins but did show homology with proteins of the natterin family. Crystal structure of the CGL1 revealed a unique homodimer in which each protomer was composed of 2 domains related by a pseudo two-fold axis. Complex structures of CGL1 with mannose molecules showed that residues have 8 hydrogen bond interactions with O1, O2, O3, O4, and O5 hydroxyl groups of mannose. The complex interactions that are not observed with other mannose-binding lectins revealed the structural basis for the strict specificity for mannose. These characteristics of CGL1 may be helpful as a research tool and for clinical applications.
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Identification, Characterization, and X-ray Crystallographic Analysis of a Novel Type of Mannose-Specific Lectin CGL1 from the Pacific Oyster Crassostrea gigas.,Unno H, Matsuyama K, Tsuji Y, Goda S, Hiemori K, Tateno H, Hirabayashi J, Hatakeyama T Sci Rep. 2016 Jul 5;6:29135. doi: 10.1038/srep29135. PMID:27377186<ref>PMID:27377186</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5idb" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Cragi]]
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[[Category: Crassostrea gigas]]
[[Category: Large Structures]]
[[Category: Large Structures]]
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[[Category: Unno, H]]
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[[Category: Unno H]]
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[[Category: Cgl1]]
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[[Category: Crassostrea giga]]
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[[Category: Lectin]]
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[[Category: Mannose]]
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[[Category: N-type]]
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[[Category: Sugar binding protein]]
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Current revision

Crystal structure of CGL1 from Crassostrea gigas, mannose-bound form (CGL1/MAN2)

PDB ID 5idb

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OCA

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