|
|
| (One intermediate revision not shown.) |
| Line 3: |
Line 3: |
| | <StructureSection load='6sl8' size='340' side='right'caption='[[6sl8]], [[Resolution|resolution]] 1.53Å' scene=''> | | <StructureSection load='6sl8' size='340' side='right'caption='[[6sl8]], [[Resolution|resolution]] 1.53Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6sl8]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_lautus"_batchelor_1919 "bacillus lautus" batchelor 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SL8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SL8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sl8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_sp._Y412MC10 Paenibacillus sp. Y412MC10]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SL8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SL8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DAB:2,4-DIAMINOBUTYRIC+ACID'>DAB</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ectA, D5F53_01655 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1401 "Bacillus lautus" Batchelor 1919])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DAB:2,4-DIAMINOBUTYRIC+ACID'>DAB</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Diaminobutyrate_acetyltransferase Diaminobutyrate acetyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.1.178 2.3.1.178] </span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sl8 OCA], [https://pdbe.org/6sl8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sl8 RCSB], [https://www.ebi.ac.uk/pdbsum/6sl8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sl8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sl8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sl8 OCA], [http://pdbe.org/6sl8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sl8 RCSB], [http://www.ebi.ac.uk/pdbsum/6sl8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sl8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A0A385TZ63_PAELA A0A385TZ63_PAELA]] Catalyzes the acetylation of L-2,4-diaminobutyrate (DABA) to gamma-N-acetyl-alpha,gamma-diaminobutyric acid (ADABA) with acetyl coenzyme A.[RuleBase:RU365045] | + | [https://www.uniprot.org/uniprot/D3EKC1_GEOS4 D3EKC1_GEOS4] Catalyzes the acetylation of L-2,4-diaminobutyrate (DABA) to gamma-N-acetyl-alpha,gamma-diaminobutyric acid (ADABA) with acetyl coenzyme A.[ARBA:ARBA00003741][RuleBase:RU365045] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
| Line 24: |
Line 23: |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Bacillus lautus batchelor 1919]] | |
| - | [[Category: Diaminobutyrate acetyltransferase]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Bremer, E]] | + | [[Category: Paenibacillus sp. Y412MC10]] |
| - | [[Category: Czech, L]] | + | [[Category: Bremer E]] |
| - | [[Category: Hoeppner, A]] | + | [[Category: Czech L]] |
| - | [[Category: Kobus, S]] | + | [[Category: Hoeppner A]] |
| - | [[Category: Richter, A A]] | + | [[Category: Kobus S]] |
| - | [[Category: Smits, S H.J]] | + | [[Category: Richter AA]] |
| - | [[Category: 4-diaminobutyrate acetyltransferase]]
| + | [[Category: Smits SHJ]] |
| - | [[Category: Acetyl coenzyme some]]
| + | |
| - | [[Category: Acetylation]]
| + | |
| - | [[Category: Chemical chaperone]]
| + | |
| - | [[Category: L-2]]
| + | |
| - | [[Category: Stress response]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
D3EKC1_GEOS4 Catalyzes the acetylation of L-2,4-diaminobutyrate (DABA) to gamma-N-acetyl-alpha,gamma-diaminobutyric acid (ADABA) with acetyl coenzyme A.[ARBA:ARBA00003741][RuleBase:RU365045]
Publication Abstract from PubMed
Ectoine is a solute compatible with the physiologies of both prokaryotic and eukaryotic cells and is widely synthesized by bacteria as an osmotic stress protectant. Because it preserves functional attributes of proteins and macromolecular complexes, it is considered a chemical chaperone and has found numerous practical applications. However, the mechanism of its biosynthesis is incompletely understood. The second step in ectoine biosynthesis is catalyzed by L-2,4-diaminobutyrate acetyltransferase (EctA; EC 2.3.1.178), which transfers the acetyl group from acetyl CoA to EctB-formed L-2,4-diaminobutyrate (DAB), yielding N-gamma-acetyl-L-2,4-diaminobutyrate (N-gamma-ADABA), the substrate of ectoine synthase (EctC). Here, we report the biochemical and structural characterization of the EctA enzyme from the thermotolerant bacterium Paenibacillus lautus (Pl). We found that (Pl)EctA forms a homodimer whose enzyme activity is highly regiospecific by producing N-gamma-ADABA but not the ectoine catabolic intermediate N-alpha-ADABA. High-resolution crystal structures of (Pl)EctA (at 1.2-2.2 A resolution) for (i) its apo form, (ii) in complex with CoA, (iii) in complex with DAB, (iv) in complex with both with CoA and DAB, and (v) in the presence of the product N-gamma-ADABA were obtained. To pinpoint residues involved in DAB binding, we probed the structure-function relationship of (Pl)EctA by site-directed mutagenesis. Phylogenomics shows that EctA-type proteins from both Bacteria and Archaea are evolutionarily highly conserved, including catalytically important residues. Collectively, our biochemical and structural findings yielded detailed insights into the catalytic core of the EctA enzyme that laid the foundation for unraveling its reaction mechanism.
The architecture of the diaminobutyrate acetyltransferase active site provides mechanistic insight into the biosynthesis of the chemical chaperone ectoine.,Richter AA, Kobus S, Czech L, Hoeppner A, Zarzycki J, Erb TJ, Lauterbach L, Dickschat JS, Bremer E, Smits SHJ J Biol Chem. 2020 Jan 22. pii: RA119.011277. doi: 10.1074/jbc.RA119.011277. PMID:31969391[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Richter AA, Kobus S, Czech L, Hoeppner A, Zarzycki J, Erb TJ, Lauterbach L, Dickschat JS, Bremer E, Smits SHJ. The architecture of the diaminobutyrate acetyltransferase active site provides mechanistic insight into the biosynthesis of the chemical chaperone ectoine. J Biol Chem. 2020 Jan 22. pii: RA119.011277. doi: 10.1074/jbc.RA119.011277. PMID:31969391 doi:http://dx.doi.org/10.1074/jbc.RA119.011277
|
