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| | ==Solution structure of the KH-QUA2 region of the Xenopus STAR-GSG Quaking protein.== | | ==Solution structure of the KH-QUA2 region of the Xenopus STAR-GSG Quaking protein.== |
| - | <StructureSection load='2bl5' size='340' side='right'caption='[[2bl5]], [[NMR_Ensembles_of_Models | 17 NMR models]]' scene=''> | + | <StructureSection load='2bl5' size='340' side='right'caption='[[2bl5]]' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[2bl5]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/African_clawed_frog African clawed frog]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BL5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BL5 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[2bl5]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Xenopus_laevis Xenopus laevis]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BL5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2BL5 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bl5 OCA], [http://pdbe.org/2bl5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bl5 RCSB], [http://www.ebi.ac.uk/pdbsum/2bl5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bl5 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2bl5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bl5 OCA], [https://pdbe.org/2bl5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2bl5 RCSB], [https://www.ebi.ac.uk/pdbsum/2bl5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2bl5 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/QKIB_XENLA QKIB_XENLA]] RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs which promotes oligodendrocyte differentiation. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of some pre-mRNA. Acts as a translational repressor (By similarity). | + | [https://www.uniprot.org/uniprot/QKIA_XENLA QKIA_XENLA] RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs which promotes oligodendrocyte differentiation. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of some pre-mRNA. Acts as a translational repressor (By similarity). Essential for notochord development.<ref>PMID:9303534</ref> |
| | == Evolutionary Conservation == | | == Evolutionary Conservation == |
| | [[Image:Consurf_key_small.gif|200px|right]] | | [[Image:Consurf_key_small.gif|200px|right]] |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: African clawed frog]] | |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Broadhurst, R W]] | + | [[Category: Xenopus laevis]] |
| - | [[Category: Guler-Gane, G]] | + | [[Category: Broadhurst RW]] |
| - | [[Category: Maguire, M L]] | + | [[Category: Guler-Gane G]] |
| - | [[Category: Nietlispach, D]] | + | [[Category: Maguire ML]] |
| - | [[Category: Raine, A R.C]] | + | [[Category: Nietlispach D]] |
| - | [[Category: Standart, N]] | + | [[Category: Raine ARC]] |
| - | [[Category: Zorn, A M]] | + | [[Category: Standart N]] |
| - | [[Category: Gsg protein]]
| + | [[Category: Zorn AM]] |
| - | [[Category: Quaking]]
| + | |
| - | [[Category: Rna binding]]
| + | |
| - | [[Category: Star protein]]
| + | |
| Structural highlights
Function
QKIA_XENLA RNA-binding protein that plays a central role in myelinization. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence. Acts by regulating pre-mRNA splicing, mRNA export, mRNA stability and protein translation. Required to protect and promote stability of mRNAs which promotes oligodendrocyte differentiation. Participates in mRNA transport by regulating the nuclear export of MBP mRNA. Also involved in regulation of mRNA splicing of some pre-mRNA. Acts as a translational repressor (By similarity). Essential for notochord development.[1]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Quaking protein belongs to the family of STAR/GSG domain RNA-binding proteins and is involved in multiple cell signalling and developmental processes in vertebrates, including the formation of myelin. Heteronuclear NMR methods were used to determine the solution structure of a 134 residue fragment spanning the KH and QUA2 homology regions of the Quaking protein from Xenopus laevis (pXqua) in the absence of RNA. The protein is shown to adopt an extended type I KH domain fold that is connected to a structured alpha-helix in the C-terminal QUA2 region by means of a highly flexible linker. A comparison with the solution structure of the related protein splicing factor 1 (SF1) indicates that most aspects of the RNA-binding interface are conserved in pXqua, although the "variable loop" region that follows the second beta-strand possesses two additional alpha-helices. The structure of pXqua provides an appropriate template for building models of important homologues, such as GLD-1 and Sam68. Measurements of the (15)N relaxation parameters of pXqua confirm that the polypeptide backbone of the QUA2 region is more dynamic than that of the KH portion, and that the C-terminal helix is partially structured in the absence of RNA. By comparison with a random coil reference state, the nascent structure in the QUA2 region is estimated to contribute 15.5kJmol(-1) to the change in conformational free energy that occurs on forming a complex with RNA. Since STAR/GSG proteins may regulate alternative splicing by competing with SF1 in the nucleus for specific branch-point sequences that signal intronic RNA, the formation of secondary structure in the QUA2 region in the unbound state of pXqua has important functional consequences.
Solution structure and backbone dynamics of the KH-QUA2 region of the Xenopus STAR/GSG quaking protein.,Maguire ML, Guler-Gane G, Nietlispach D, Raine AR, Zorn AM, Standart N, Broadhurst RW J Mol Biol. 2005 Apr 29;348(2):265-79. PMID:15811367[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Zorn AM, Krieg PA. The KH domain protein encoded by quaking functions as a dimer and is essential for notochord development in Xenopus embryos. Genes Dev. 1997 Sep 1;11(17):2176-90. PMID:9303534
- ↑ Maguire ML, Guler-Gane G, Nietlispach D, Raine AR, Zorn AM, Standart N, Broadhurst RW. Solution structure and backbone dynamics of the KH-QUA2 region of the Xenopus STAR/GSG quaking protein. J Mol Biol. 2005 Apr 29;348(2):265-79. PMID:15811367 doi:10.1016/j.jmb.2005.02.058
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