This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
6y34
From Proteopedia
(Difference between revisions)
m (Protected "6y34" [edit=sysop:move=sysop]) |
|||
| (One intermediate revision not shown.) | |||
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Streptavidin wildtype with a biotC5-1 cofactor - an artificial iron hydroxylase== | |
| + | <StructureSection load='6y34' size='340' side='right'caption='[[6y34]], [[Resolution|resolution]] 1.31Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6y34]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Y34 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Y34 FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.307Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=O7Q:biotC5-1+cofactor'>O7Q</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6y34 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6y34 OCA], [https://pdbe.org/6y34 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6y34 RCSB], [https://www.ebi.ac.uk/pdbsum/6y34 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6y34 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin). | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The selective hydroxylation of C-H bonds is of great interest to the synthetic community. Both homogeneous catalysts and enzymes offer complementary means to tackle this challenge. Herein, we show that biotinylated Fe(TAML)-complexes (TAML = Tetra Amido Macrocyclic Ligand) can be used as cofactors for incorporation into streptavidin to assemble artificial hydroxylases. Chemo-genetic optimization of both cofactor and streptavidin allowed optimizing the performance of the hydroxylase. Using H2O2 as oxidant, up to approximately 300 turnovers for the oxidation of benzylic C-H bonds were obtained. Upgrading the ee was achieved by kinetic resolution of the resulting benzylic alcohol to afford up to >98% ee for (R)-tetralol. X-ray analysis of artificial hydroxylases highlights critical details of the second coordination sphere around the Fe(TAML) cofactor. | ||
| - | + | Enantioselective Hydroxylation of Benzylic C(sp(3))-H Bonds by an Artificial Iron Hydroxylase Based on the Biotin-Streptavidin Technology.,Serrano-Plana J, Rumo C, Rebelein JG, Peterson RL, Barnet M, Ward TR J Am Chem Soc. 2020 Jun 17;142(24):10617-10623. doi: 10.1021/jacs.0c02788. Epub, 2020 Jun 3. PMID:32450689<ref>PMID:32450689</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6y34" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Avidin 3D structures|Avidin 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Streptomyces avidinii]] | ||
| + | [[Category: Barnet M]] | ||
| + | [[Category: Peterson RL]] | ||
| + | [[Category: Rebelein JG]] | ||
| + | [[Category: Rumo C]] | ||
| + | [[Category: Serrano-Plana J]] | ||
| + | [[Category: Ward TR]] | ||
Current revision
Streptavidin wildtype with a biotC5-1 cofactor - an artificial iron hydroxylase
| |||||||||||
